| 电离辐射对EL-4细胞凋亡与坏死的影响 |
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| 引用本文： | 薛丽香,金光辉,付士波,苏旭. 电离辐射对EL-4细胞凋亡与坏死的影响[J]. 中华放射医学与防护杂志, 2001, 21(1): 24-26 |
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| 作者姓名： | 薛丽香 金光辉 付士波 苏旭 |
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| 作者单位： | 1. 白求恩医科大学卫生部放射生物学重点实验室 长春，130021
2. 卫生部工业卫生实验所 |
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| 基金项目： | 卫生部科学研究基金资助项目(98-1-202) |
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| 摘 要： | 目的 研究不同剂量电离辐射对EL－4细胞凋亡和细胞坏死的影响。方法 采用PI和Hoechst 33342双染、流式细胞术检测。结果 实验结果表明：给予50－200mGy低剂量照射后6h，EL－4细胞凋亡和坏死较对照组没有升高，而给予1．0－8．0Gy大剂量X射线照射后6h,细胞凋亡较对照组有明显升高，4．0Gy以上剂量，细胞坏死显著升高。结论 一定剂量的电离辐射不仅可以诱导凋亡的产生，而且可直接导致细胞坏死，即细胞的死亡模式与受照剂量有关。
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| 关 键 词： | 电离辐射 细胞凋亡 细胞坏死 EL－4 |
| 收稿时间： | 2000-02-12 |
| 修稿时间： | 2000-02-12 |
Studies on apoptosis and necrosis of EL-4 cells after irradiation with different doses of X-rays |
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| XUE lixiang,JIN Guanghui,FU Shibo. Studies on apoptosis and necrosis of EL-4 cells after irradiation with different doses of X-rays[J]. Chinese Journal of Radiological Medicine and Protection, 2001, 21(1): 24-26 |
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| Authors: | XUE lixiang JIN Guanghui FU Shibo |
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| Affiliation: | XUE lixiang,JIN Guanghui,FU Shibo,et al.MH Radiobiology Research Unit,Norman Bethune University of Medical Sciences,Changchun 130021,China |
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| Abstract: | Objective To study the changes in apoptosis and necrosis of EL|4 cells following irradiation with different doses of X|rays. Methods The cells were stained by PI and Hoechst 33342 six hours after irradiation,and the changes in apoptosis and necrosis were analyzed by flow cytometry. Results It was found that apoptosis and necrosis increased significantly after high dose irradiation. Conclusion The results indicate that irradiation with certain doses can not only induce apoptosis but also cause necrosis at the same time. |
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| Keywords: | Irradiation Apoptosis Necrosis |
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