抗肿瘤中药对小鼠U14宫颈癌细胞多种酶活性的影响

目的：探讨抗肿瘤中药对癌细胞的繁殖起重要作用的ACPase、ATKPase、SDH、G－6－PDH在U14宫颈癌细胞中活性的影响。方法：荷瘤小鼠在第7d用药后6h处死，取腹水癌细胞涂片，固定显示，用细胞分光光度计测定酶活性。结果：中药合剂实验组细胞内ACPase、ATPase、SDH、G－6－PDH酶活性比生理盐水对照组显著降低（P＜0．01），分别下降60％、40％、56％、70％，与环磷酰胺组比无显著性差异（P＞0．05）。结论：抗肿瘤中药合剂能显著地抑制U14宫颈癌细胞内ACPase、ATPase、SDH、G－6－PDH活性，呈现抑瘤作用。

Effect of Antitumor Chinese Medicine on Activities of ATPase, ACPase, SDH,G-6-PDH in U14 Cervix Cancer Cells

Objective: To test the effect of antitumor Chinese medicine on activities of ATPase, ACPase, SDH,G-6-PDH in U 14 cervix cancer cells, those enzymes are crucial for cancer cell proliferation. Methods: Mice with U 14 ascites carcinoma were killed by neck luxation, U 14 cells in ascites were aspitrated out, smeared on slices, fixed, color-developed according to the instruction of kits, color-measured by using cytospectrophotometer and intracelluar enzymatic activities were eventurally resulted via statistical calculation. Results: The activities of ATPase, ACPase, SDH,G-6-PDH were significantly decreased (P<0 01) by 60%, 40%,56%,and 70% respectively, compared with normal control group. The difference between Chinese medicine group and cyclophosphamide group showed no significance (P>0 05). Conclusion: The antitumor Chinese medicine can significantly inhibite the activites of ATPase, ACPase, SDH,G-6-PDH in U 14 cervix cancer cells,thus exert a powerful suppression action on tumor cells and reasonably be an ideal antitumor medicine.