氨基胍对糖尿病大鼠肾损害的治疗作用

目的通过探讨氨基胍（AG）对早期糖尿病（DM）大鼠一氧化氮（NO）、一氧化氮合酶（ni-tric oxi desynthase,NOS）活性及24h尿蛋白排泄量（24hUPE）的影响,了解氨基胍对糖尿病肾病的治疗作用。方法健康清洁级Wistar大鼠40只,检测24hUPE、血清NO水平、总一氧化氮合酶（total nitric oxide synthase,tNOS）和诱导型一氧化氮合酶（inducible nitric oxide synthase,iNOS）及结构型一氧化氮合酶（constructive nitric oxide synthase,cNOS）活性等5项指标后,用链脲佐菌素（Streptozotocin,STZ）60mg/kg制备成糖尿病大鼠模型,将糖尿病鼠随机分为糖尿病对照组、氨基胍组（AG组）。于8周末时再检测大鼠的上述5项指标并进行统计分析。结果①与造模前比较,DM对照组在8周末时24hUPE、NO和iNOS升高（P〈0.01,P〈0.05）;AG组24hUPE增加（P〈0.01）,tNOS、iNOS、cNOS降低（P〈0.01,P〈0.05）。②与DM对照组比较,8周末时AG组5项指标均下降（P〈0.05）。结论在糖尿病肾病（DN）的发展进程中,早期阶段应用AG可通过降低血NOS活性、NO生成量及其他机制,使24hUPE减少,减轻肾脏的损害。

The therapeutic effects of aminoguanidine on renal injury of diabetic rats

Objective To investigate the effect of aminoguanidine（AG） on serum nitric oxide （NO）,nitric oxide synthase（NOS） activity and renal function of diabetic rats,To study the therapeutic effects of AG on diabetic nephropathy. Methods Forty healthy Wistar rats were involved in the study. The 24 hr urinary protein excretion （24 h UPE）,serum levels of NO,total nitric oxide synthase （tNOS） activity,inducible nitric oxide synthase （iNOS） activity and constructive nitric oxide synthase （cNOS） activity were detected to the rats. Streptozotocin （STZ,60 mg/kg） was administrated in the rats. The STZ diabetic rats were randomly divided into diabetes control group and AG group. At the end of 8 weeks after STZ administration,the above five indexes of the rats were detected and statistical analysis was carried out. Results ① compared with normal rats,24 h UPE,iNOS and NO of the rats in DM control group increased significantly （P〈0.01,P〈0.05）,In the rats of AG group,24 h UPE increased while both tNOS,iNOS and cNOS decreased （P〈0.01,P〈0.05）. ②Compared with the diabetes control group ,the five indexes were significantly decreased in AG group （P〈0.05）. Conclusion In the progress of DM,the early administration of aminoguanidine can effectively reduce the twenty-four-hour urinary protein excretion and renal injury by decreasing the NOS activity,NO level and other more important mechanisms.