A microsatellite-based,physically anchored linkage map for the gray,short-tailed Opossum (<Emphasis Type="Italic">Monodelphis domestica</Emphasis>) |
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Authors: | Paul B Samollow Nicolas Gouin Pat Miethke Susan M Mahaney Margaret Kenney John L VandeBerg Jennifer A Marshall Graves Candace M Kammerer |
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Institution: | (1) Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843-4458, USA;(2) Department of Genetics and Southwest National Primate Research Center, Southwest Foundation for Biomedical Research, San Antonio, TX 78245-0549, USA;(3) Comparative Genomics Research Group and ARC Centre for Kangaroo Genomics, Research School of Biological Sciences, The Australian National University, Canberra, ACT 2061, Australia;(4) Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 15261, USA |
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Abstract: | The genome of the gray, short-tailed opossum, Monodelphis domestica, will be the first of any marsupial to be fully sequenced. The utility of this sequence will be greatly enhanced by construction
and integration of detailed genetic and physical maps. Therefore, it is important to verify the unusual recombinational characteristics
that were suggested by the ‘first-generation’ M. domestica linkage map; specifically, very low levels of recombination and severely reduced female recombination, both of which are
contrary to patterns in other vertebrates. We constructed a new linkage map based on a different genetic cross, using a new
and much larger set of map markers, and physically anchored and oriented the linkage groups onto chromosomes via fluorescence
in-situ hybridization mapping. This map includes 150 loci in eight autosomal linkage groups corresponding to the eight autosome pairs,
and spans 86–89% of the autosomal genome. The sex-averaged autosomal map covers 715 cM, with a full-length estimate of 866 cM;
the shortest full-length linkage map reported for any vertebrate. The sex-specific maps confirmed severely reduced female
recombination in all linkage groups, and an overall F/M map ratio = 0.54. These results greatly extend earlier findings,
and provide an improved microsatellite-based linkage map for this species.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | linkage Monodelphis domestica physical mapping recombination sex-specific |
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