抗坏血酸与DL—α—生育酚对人肝癌细胞增殖与再分化的作用

目的:测定抗坏血酸(AA)和DL-α-生育酚(αT)联合处理对人肝癌细胞增殖与再分化的作用。方法:维甲酸(RA)为阳性对照。用细胞表面电荷、生化变化和软琼脂细胞生长等指标测定细胞分化和增殖。结果:用AA 4 mmol·L~(-1)和αT 1 mmol·L~(-1)联合处理后,肝癌细胞的生长和分裂指数显著下降,增殖抑制率达61.3％。与恶化有关的指标显著减轻,如细胞表面电荷明显降低,电泳率从1.63 μm·s~(-1)·V~(-1)·cm~(-1)降低到0.89,甲胎蛋白由300μg·g~(-1)(protein)降到80,γ-谷氨酰转肽酶活性由0.81 U·g~(-1)(protein)降到0.201。与分化相关的酪氨酸-α-酮戊二酸转氨酶活性显著上升,由10.6μmol·g~(-1)(protein)升高到45,细胞克隆形成力降低96.6％。结论:AA和αT合用能够抑制人肝癌细胞增殖,诱导分化,并逆转恶性表型。

Effects of ascorbic acid and DL-alpha-tocopherol on human hepatoma cell proliferation and redifferentiation

AIM: To examine the effects of ascorbic acid (AA) and DL-alpha-tocopherol (alpha T) on the proliferation and redifferentiation of human hepatoma cell. METHODS: Choosing an all-trans retinoic acid (RA) as a positive control, cell surface charge, biochemical changes, and cell growth in soft agar were measured. RESULTS: After treatment with AA 4 mmol.L-1 and alpha T 1 mmol.L-1 together, the growth curve and mitotic index of human hepatoma cells decreased remarkably, the cellular growth inhibitory rate amounted to 61.3%. The indices related to cell malignancy alleviated significantly; cell surface charge decreased, the electrophoresis rate dropped from 1.64 to 0.89 microns.s-1.V-1.cm-1, the average value of alpha-fetoprotein (alpha-FP) content decreased from 300 to 80 micrograms.g-1 (protein), and gamma-glutamyl-transpeptidase (gamma-GT) activity decreased from 0.81 to 0.201 U.g-1(protein). The index related to cell differentiation increased significantly, such as the average level of tyrosine-alpha-ketoglutarate transaminase activity increased from 10.6 to 45 micrograms.g-1 (protein), and the colonogenic potential decreased by 96.6%. CONCLUSION: AA and alpha T combination inhibited human hepatoma cell proliferation, induced redifferentiation, and reversed its malignant phenotypic characteristics.