他克林优先拮抗4种莨菪类生物碱引起的小鼠学习障碍(英文)

目的:比较他克林(tacrine,50μmol·kg~(-1),sc)拮抗莨菪类生物碱(0.05-50μmol·kg~(-1),ip)所致学习记忆障碍。方法:用两次性开阔和回避反应研究,小鼠于第一次实验前30min给药。结果:他克林对东莨菪碱(Sco)0.05-50μmol·kg~(-1)或阿托品(Atr)5-50μmol·kg~(-1)引起的学习和记忆障碍均有拮抗作用,以拮抗学习障碍最明显。他克林能改善樟柳碱(AT_3)和山莨菪碱(Ani)5-50μmol·kg~(-1)所致学习损伤,但不能拮抗其记忆损伤。Sco 0.5-50μmol·kg~(-1),AT_3和Atr5-50μmol·kg~(-1)完全拮抗,Ani 50μmol·kg~(-1)部分拮抗他克林引起的自主活动减少。结论:他克林优先改善莨菪类药物引起的被动回避反应学习障碍。

Learning deficits induced by 4 belladonna alkaloids are preferentially attenuated by tacrine

AIM: To examine the antagonism of tacrine on the amnesic effects of scopolamine (Sco), anisodine (AT3), atropine (Atr), and anisodamine (Ani). METHODS: Cognitive functions and locomotor activities were determined using two sessions of step-through and open-field tests, respectively. Mice were injected with one of the belladonna alkaloids (0.05-50 mumol.kg-1, i.p.) and tacrine (50 mumol.kg-1, s.c.) 30 min before the first session. RESULTS: Tacrine completely blocked the avoidance-learning deficit caused by Sco 0.5 mumol.kg-1, AT3 and Atr 5 mumol.kg-1, or Ani 50 mumol.kg-1. But tacrine partly antagonized the learning deficit induced by Sco 5-50 mumol.kg-1 or Atr and AT3 50 mumol.kg-1. The avoidance-memory deficit caused by Sco 0.05-5 mumol.kg-1 or Atr 5 mumol.kg-1 was completely or partly attenuated by tacrine, which did not antagonize the memory deficit elicited by Sco and Atr 50 mumol.kg-1, AT3 5 and 50 mumol.kg-1, and Ani 50 mumol.kg-1. During the acquisition, the locomotor activity of the mice was inhibited by tacrine. This reduction was completely antagonized by Sco 0.5-50 mumol.kg-1, AT3 5-50 mumol.kg-1, Atr 5-50 mumol.kg-1, and only partly antagonized by AT3 and Atr 0.5 mumol.kg-1 or Ani 50 mumol.kg-1. CONCLUSION: Compared with the avoidance-memory deficit, the avoidance-learning deficit caused by belladonna alkaloids is more preferentially attenuated by tacrine.