哮喘小鼠脾细胞中粒细胞型髓源性抑制细胞和Th17细胞水平的变化

目的: 探索髓源性抑制细胞（myeloid derived suppressor cells，MDSCs）、Th17及调节性T细胞（Treg）在实验性哮喘小鼠脾脏中的水平。方法: 将6～8周BALB/c雄性小鼠随机分为PBS组和模型组；用卵清蛋白建立哮喘小鼠模型，采用酶联免疫吸附法(ELISA)检测小鼠血清中卵清蛋白特异性的IgE；采用HE染色和PAS染色观察小鼠肺组织的病理变化；用流式细胞术检测脾细胞中Th17、Treg、MDSCs的比例；采用免疫组化观察小鼠肺组织骨髓分化抗原（Gr 1）、白介素17（IL 17）、叉头样转录因子3（Foxp3）的表达水平。结果： 与PBS组相比，模型组小鼠气道炎性细胞增多，黏液分泌增加，血清IgE水平明显增高，脾细胞中Th17细胞的比例增加、Treg细胞比例减少；MDSCs中的粒细胞型亚群（G-MDSCs）的比例增加；同时肺组织中IL-17、Gr-1高表达，Foxp3低表达。结论： G MDSCs、Th17细胞比例增加，Treg细胞比例减少可能参与小鼠哮喘的发病。

Changes of granulocytic myeloid-derived suppressor cell and Th17 cells in spleen cells of asthmatic mice

Objective: To explore the level of myeloid-derived suppressor cells(MDSCs), Th17 and Treg cells in spleen cells of asthmatic mice. Methods: BALB/c male mice at age of 6-8 weeks were randomly divided into two groups: PBS group and model group. The group of asthmatic mice was established with OVA, and the ova-specific IgE in the mouse serum was detected by enzyme-linked immunosorbent assay (ELISA). HE staining and PAS staining were used to observe the pathological change in lung tissues. The levels of Th17, Treg and MDSCs in splenocyte were detected by flow cytometry. Immunohistochemical staining was used to observe the expression of Gr-1, IL-17 and Foxp3 in lung tissues of mice. Results: Compared with PBS group, airway inflammatory cells and mucus secretion were increased in the model group and serum IgE level was significantly increased. The proportion of Th17 cells in spleen cells increased and that of Treg cells decreased. The percentage of G-MDSCs subset in MDSCs was increased. Meanwhile, IL-17 and Gr-1 were highly expressed and Foxp3 was low in lung tissue of model group. Conclusion: High proportion of G-MDSCs and Th17 cells, and low proportion of Treg may be contributed to pathogenesis of asthmatic mice.