非编码 RNA GlmY 对伤寒沙门菌生物膜形成的影响

目的: 探究非编码 RNA GlmY 对伤寒沙门菌生物膜形成的影响及其分子机制。方法: 用自杀质粒pGMB151介导的同源重组方法，制备 glmY 缺陷株；用96孔微量板结晶紫染色法检测各菌株生物膜形成情况；通过qRT PCR分析glmY 缺陷株及野生株中生物膜形成相关基因的mRNA表达水平。结果： 成功制备glmY缺陷株；细菌生物膜形成实验表明，与野生株相比，glmY缺陷株生物膜形成能力明显下降；qRT PCR分析结果显示，GlmY缺失后卷曲菌毛合成相关基因csgD和csgA的mRNA表达水平明显下调。结论： 伤寒沙门菌非编码 RNA GlmY可通过上调csgD和csgA的表达促进卷曲菌毛合成，进而促进生物膜的形成。

Effect of non-coding RNA GlmY on biofilm formation of Salmonella enterica serovar Typhi

Objective: To investigate the effect of non-coding RNA GlmY on the biofilm formation of Salmonella enterica serovar Typhi (S. Typhi) and its potential molecular mechanisms.Methods: The homologous recombination induced by the suicide plasmid pGMB151 was used to generate the glmY deleted mutant of S. Typhi. The bacterial biofilm formation in 96-microwell plate was detected by crystal violet staining. qRT-PCR was used to analyze the mRNA expression levels of biofilm formation-related genes in glmY deleted mutant and wild-type strain. Results: The glmY deletion mutant strain was successfully constructed. Compared with the wild-type strain, the glmY deletion mutant showed significantly weaker ability of biofilm formation. The qRT-PCR results showed that mRNA level of csgD and csgA was significantly decreased after deletion of GlmY. Conclusion: The non-coding RNA GlmY may enhance the biofilm formation of S. Typhi by up-regulating the expression of csgD, csgA and Curli synthesis.