组蛋白去乙酰化酶1及基质金属蛋白酶-2在肝细胞癌组织中的表达及意义

目的探讨组蛋白去乙酰化酶1(HDAC1)及基质金属蛋白酶-2(MMP-2)在肝细胞癌(HCC)患者肝组织中的表达及其临床意义。方法采用免疫组织化学方法检测42例HCC和相应的癌旁组织中HDAC1及MMP-2蛋白的表达,并分析其与临床病理参数的关系。结果 HCC组织中,HDAC1及MMP-2蛋白的表达明显高于癌旁组织(P<0.01,P<0.05);HDAC1蛋白的表达与患者性别、年龄、肿瘤大小、AFP浓度、肿瘤分化程度、脉管癌栓无关联(P>0.05),与肿瘤TNM分期相关联(P<0.05);MMP-2蛋白的表达与患者性别、年龄、肿瘤大小、AFP浓度、肿瘤分化程度无关联(P>0.05),与脉管癌栓、肿瘤TNM分期相关联(P<0.05,P<0.01);HCC组织中,HDAC1及MMP-2蛋白的表达呈正相关(P<0.01)。结论 HDAC1及MMP-2在HCC患者肝组织中表达有一定的肿瘤特异性,二者的高表达提示HCC已属晚期。

Expression of histone deacetylase 1 and matrix metalloproteinase-2 in hepatocellular carcinoma and its significance

Objective To study the expression of HDAC1 and MMP-2 in liver tissues of patients with hepatocellular carcinoma(HCC) and its significance.Methods Immunohistochemical analysis was used to detect the expression of HDAC1 and MMP-2 in 42 cases of HCC.Results The positive rate of HDAC1 and MMP-2 was significantly higher in HCC than that in para-cancerous tissues(P<0.01,P<0.05),the expression of HDAC1 was not associated with patients′ gender,age,tumor size,alpha fetoprotein,histological grade and vessel embolus(P>0.05),but was significantly associated with patients′ TNM stage(P<0.05),the expression of MMP-2 was not associated with patient gender,age,tumor size,alpha fetoprotein,histological grade(P>0.05),but was significantly associated with patient vessel embolus and TNM staging(P<0.05,P<0.01),HDAC1 was positively correlated with MMP-2 expression in HCC.Conclusion In HCC tissues,increased HDAC1 and MMP-2 expression may associate to the hepatocarcinogenesis,and may indicate the status of advanced TNM stage.