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| 大蒜素对大鼠肝星状细胞基质金属蛋白酶抑制剂-1表达的影响 | |
| 引用本文： | 周晓明,刘金玲,王璐璐,郑勇,刘清华,张雅洁. 大蒜素对大鼠肝星状细胞基质金属蛋白酶抑制剂-1表达的影响[J]. 广东医药学院学报, 2013, 0(6): 656-659 |
| 作者姓名： | 周晓明 刘金玲 王璐璐 郑勇 刘清华 张雅洁 |
| 作者单位： | [1]广东药学院病理教研室,广东广州510006;广州医科大学病理教研室广东广州510000 [2]广州医科大学病理教研室,广东广州510000 [3]石河子大学医学院第一附属医院消化内科,新疆石河子832008 [4]石河子大学医学院第一附属医院社区卫生服务中心。新疆石河子832008 ,新疆石河子832008 [5]石河子大学医学院第一附属医院肿瘤内科,新疆石河子832008 |
| 基金项目： | 国家自然科学基金（81060274）;石河子大学科学技术研究发展计划项目（RCZX201025） |
| 摘 要： | 目的 观察大蒜素对大鼠肝星状细胞（HSC-T6）增殖及细胞外基质降解酶-金属蛋白酶抑制剂-1（TIMP-1）的影响,探讨大蒜素抗肝纤维化的作用及其机制.方法 体外培养大鼠肝星状细胞株HSC-T6.MTT法检测大蒜素作用下对大鼠肝星状细胞增殖的影响、Real-time RT-PCR检测肝星状细胞的TIMP-1mRNA水平、Western blot检测肝星状细胞的TIMP-1蛋白的表达水平.结果 HSC-T6大蒜素处理组与空白对照组比较,增殖抑制率明显提高,差异有统计学意义（P ＜0.01）;TIMP-1的mRNA及蛋白表达水平均明显下降,并且大蒜素浓度增高时,TIMP-1的mRNA及蛋白表达水平则逐渐下降,大蒜素高浓度（24μg/mL）组对TIMP-1的抑制作用明显高于空白对照组,差异有统计学意义（P＜0.05）.结论 大蒜素对HSC-T6增殖和TIMP-1的表达均有抑制作用,为临床开发抗肝纤维化的新药物提供了理论基础. |
| 关 键 词： | 大蒜素 肝星状细胞 细胞增殖 金属蛋白酶抑制剂-1 |
Effect of allicin on the expression of TIMP-1 in rat hepatic stellate cells |
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| ZHOU Xiaoming,LIU Jinling,WANG Lulu,ZHENG Yong,LIU Qinghua,ZHANG Yajie. Effect of allicin on the expression of TIMP-1 in rat hepatic stellate cells[J]. , 2013, 0(6): 656-659 | |
| Authors: | ZHOU Xiaoming LIU Jinling WANG Lulu ZHENG Yong LIU Qinghua ZHANG Yajie |
| Affiliation: | 1. Department of Pathology, Guangclong Pharmaceutical University, Guangzhou 510006, China; 2. Department of Pathology, Guangzhou Medical University, Guangzhou 510000, China ; 3. Department of Digestive System Diseases, The First Affiliated Hospital, Medical College, Shihezi University, Shihezi , 832008, China ; 4. Center of Community Health Services, The First Affiliated Hospital, Medical College, Shihezi University, Shihezi , 832008, China ; 5. Department of Medical Oncology , The First Affiliated Hospital, Medical College, Shihezi University, Shihezi , 832008, China) |
| Abstract: | Objective To observe the effect of allicin on rat hepatic stellate ceils （HSC-T6） proliferation and tissue inhibitor of metalloproteinase-1 （TIMP-1） expression, and its antifibrotic activity. Methods HSC-T6 cells were cultured in vitro. MTT assay was used to measure the proliferation of HSC-T6 cells. Real-time PCR and western blot were used to detect the expression of TIMP-1 mRNA and protein. Results Compared with the control group, the proliferation of HSC-T6 cells was inhibited when treated with allicin （P 〈 0.01 ）. Meantime, the expression of TIMP-1 mRNA and protein was significantly decreased in alliein-treatment group, especially when the concentration of allicin was 24 μg/mL（ P 〈 0.05 ）. Conclusion Allicin plays an important role in inhibition of HSC-T6 cells proliferation and TIMP-1 expression, which may provide an evidence for anti-hepatic fibrosis drugs research. |
| Keywords: | allicin hepatic stellate cells cell proliferation tissue inhibitor of metalloproteinase-1 |
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