UPLC法测定“柴胡-白芍”药对提取物的3种皂苷类成分

目的建立同时测定“柴胡-白芍”药对提取物中芍药苷、柴胡皂苷a及柴胡皂苷d的超高效液相色谱法。方法采用WatersAcquityUPLC系统；色谱柱：Water sXSELECT^TM HSST3（2：1mm×100mm，2．5μm）；柱温：30℃；以0．1％（体积分数）H，PO4水溶液和乙腈为流动相进行梯度洗脱；流速：0．4mL／min；检测波长：210nm。结果芍药苷、柴胡皂苷a及柴胡皂苷d3种成分基本分离，线性关系良好。芍药苷的线性范围为0．1616～1．616μg（r=1．0000），平均回收率为100．8％；柴胡皂苷a的线性范围为0．0508～0．508μg（r=0．9996），平均回收率为99．4％；柴胡皂苷d的线性范围为0．0538—0．538μg（r=0．9998），平均回收率为101．2％。结论本方法简便、快捷、准确、重复性好，可实现柴胡一白芍药对提取物的多指标质量控制。

Determination the contents of 3 saponins in the extract from couplet medicines of Radix Bupleuri-Paeoniae Alba by UPLC

Objective To establish an UPLC method for the simultaneous determination of paeoniflorin, saikosaponin a and saikosaponin d in the extract from couplet medicines of Radix Bupleuri-Paeoniae Alba. Methods The chromatographic separation was performed on Waters XSELECTTM HSS T3 column （2.1 mm × 100 mm,2.5 μm） ,and the column temperature was maintained at 30 ℃. The mobile phase consisted of 0.1% phosphoric acid aqueous solution-acetonitrile with gradient elution at the flow rate of 0.4 mL/min. The UV detection wavelength was 210 nm. Results Good resolutions and linearity could be observed among paeoniflorin, saikosaponin a and saikosaponin d. The linear range for paeoniflorin was 0. 161 6 - 1. 616 p,g （r = 1. 000 0） ,with the average recovery of 100. 8% ; The linear ranges for saikosaponin a was 0. 050 8 - O. 508 μg （r=0. 999 6）,with the average recovery of 99.4%; The linear ranges for saikosapouin d was 0. 053 8 - 0. 538 μg （ r = 0. 999 8 ）, with the average recovery of 101.2%. Conclusion The method is rapid, simple, accurate and reproducible, which can be used in the multi-index quality control in the extract of couplet medicines of Radix Bupleuri-Paeoniae Alba.