不同来源的杭菊药材HPLC指纹图谱研究

目的 优化杭菊高效液相色谱指纹图谱分析方法,建立杭菊黄酮类及酚酸类成分指纹图谱.方法 色谱柱为Kromasil 100-5 C18柱;流动相为乙腈-0.1％（体积分数）H3P04溶液,梯度洗脱;检测波长为350nm;柱温为35℃;流速为1.0mL·min-1.结果 不同来源的14批杭菊样品均标示出21个共有峰,其中有15个黄酮类成分共有峰,6个酚酸类成分共有峰;利用中药色谱指纹图谱相似度评价软件进行相似度评价,14批样品的相似度为0.926 ～0.998,提示杭菊质量较稳定.结论 本方法准确可靠、重复性好,为杭菊质量控制提供了依据.

Study on HPLC fingerprint of Flos Chrysanthem of different sources

Objective To develop an HPLC method for the fingerprint analysis on flavonoids and phenolic acids from Flos Chrysanthem （HangJu）. Methods HPLC was performed with the Kromasil 100-5 C ls column, and the acetonitrile-0.1% phosphoric acid solution （gradient elution） was employed as a mobile phase. The detection wavelength was 350 nm,and column temperature was 35℃. The flow rate was 1.0 mL min-1. Results 21 common peaks were identified from 14 batches of Flos Chrysattthem, including 15 flavonoids and 6 phenonic acids. The similarity was evaluated on the Similarity evaluation system for chromatographic fingerprint of Traditional Chinese Medicine. The similarities of 14 batches were 0. 926 - 0. 998, which indicated that the quality of Flos Chrysanthem was relatively stable. Conclusion The method is accurate and reproducible, which could be used for the quality control of Flos Chrysanthem.