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| FISH技术检测宫颈病变中hTERC基因的表达及意义 | |
| 引用本文： | 晁宏图,冯晓杰,马杰,孙淼淼,罗艳林,魏丽惠,王莉.FISH技术检测宫颈病变中hTERC基因的表达及意义[J].河南医学研究,2010,19(3). |
| 作者姓名： | 晁宏图 冯晓杰 马杰 孙淼淼 罗艳林 魏丽惠 王莉 |
| 作者单位： | 1. 河南省肿瘤医院,妇瘤科,河南,郑州,450008 2. 河南省肿瘤医院,病理科,河南,郑州,450008 3. 北京大学人民医院,北京,100044 |
| 摘 要： | 目的:检测宫颈癌和宫颈上皮内瘤变中的hTERC基因的扩增情况。方法:采用双色荧光原位杂交技术(FISH)检测100例宫颈病变脱落细胞中的hTERC基因表达。结果:良性细胞学病变、不典型鳞状细胞(ASC)、低度鳞状上皮内瘤变(LSIL)、高度鳞状上皮内瘤变(HSIL)脱落细胞hTERC基因阳性表达率分别为9.09%、25.00%、54.29%、91.67%。慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ和宫颈癌脱落细胞hTERC基因阳性表达率分别为6.67%、14.29%、64.71%、75.00%、90.24%。结论:慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ到宫颈癌的hTERC基因阳性表达率逐渐增高,宫颈低度病变与高度病变hTERC基因检测可成为临床检查宫颈癌的新手段。 |
| 关 键 词： | 荧光原位杂交 hTERC基因 宫颈癌 宫颈上皮内瘤变 |
Expression and significance of hTERC gene by FISH detection in cervical lesions |
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| CHAO Hong-tu,FENG Xiao-jie,MA Jie,SUN Miao-miao,LUO Yan-lin,WEI Li-hui,WANG Li.Expression and significance of hTERC gene by FISH detection in cervical lesions[J].Henan Medical Research,2010,19(3). | |
| Authors: | CHAO Hong-tu FENG Xiao-jie MA Jie SUN Miao-miao LUO Yan-lin WEI Li-hui WANG Li |
| Institution: | CHAO Hong-tu1,FENG Xiao-jie1,MA Jie2,SUN Miao-miao2,LUO Yan-lin1,WEI Li-hui3,WANG Li1.(1.Department of Gynecologic Oncology,Henan Tumor Hospital,Zhengzhou 450008,China,2.Department of Pahtology,3.People's hospital of Peking University,Beijing 100044,China) |
| Abstract: | Objective: To detect the human telomerase(hTERC)amplication of cervical carcinoma and CIN.Methods: hTERC expression were detected by FISH from 100 cases cervical intraepithelial neoplasia.Results: Rate of expression of hTERC in chronic cervicitis,atypical squamous cells(ASC),low-grade squamous intraepithelial lesion(LSIL),high-grade squamous intraepithelial lesion(HSIL) were 9.09%,25.00%,54.29%,and 91.67%, respectively. Expression rate of hTERC in chronic cercicitis,CINⅠ,CINⅡ,CINⅢ and cervical cancer were 6.67%,14.29%,64.71%,75.00%,and 90.24%,respectively.Conclusion: The positive of hTERC expression is closely related to cervical disease level.FISH may be an adjunct to cytology screening, especially high-risk patients. |
| Keywords: | fluorescence in situ hybridization hTERC gene cervical carcinoma cervical intraepithelial neoplasia |
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