胶体金免疫渗滤法快速检测幽门螺杆菌血清抗体的研究和应用

目的建立一种简便、快速、准确的检测胃幽门螺杆菌(H.pylori)抗体的新型血清学诊断方法。方法用胶体金标记技术与渗滤法相结合，建立了快速胶体金免疫渗滤法(DIGFA)，与ELISA对比检测145份H.pylori感染血清，并与病理学检查对比检测108份H.pylori血清。做阻断试验和IgG标准品最小蛋白含量的检出观察，对其敏感性、特异性做了分析并调查了216例不同年龄组的人群感染率。结果本法与ELISA相比，两者符合率为95.1％；与病理组织染色检查比较，两者符合率为88.8％。DIGFA可检出IgG标准品的最小蛋白量为10μg

Study and application ofimmunogold filtration assay for detecting serum antibodies against helicobacter pylori

Objectives　To establish a simple,quick,accurate serodiagnostic assay for detecting serum antibodies against Helicobacter pylori(H.pylori).Methods　We established quick immunocolloidal gold filtration assay(DIGFA)by combining colloidal gold labelling technique with filtration method.145 sera from cases with H.pylori infection were detected by DIGFA,and the results were compared with that of ELISA and pathological examinations.In addition,blocking test and human IgG protein detection were conducted.The sensitivity,specificity of DIGFA were analysed.Besides,DIGFA was applied to investigate the H.pylori infection situation among persons of different ages.Results　The coincidence rate reached 95.1% compared with the ELISA,and it was 88.8% compared with pathological the examination.The detectable minimum amount of IgG was l0μg/mL.The antibodies detected by DIGFA proved to be specific antibodies by blocking test.The human H.pylori infection rate among 216 persons reached 42.5% by DIGFA.Conclusions　DIGFA can be applied effectively in clinical diagnosis and epidemiological survey.