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Simultaneous Determination of Three Flavone Constituents in Ophiopogonis Radix by HPLC Method

??OBJECTIVE To establish an HPLC method for simultaneous determination of methylophiopogonone A, methylophiopogonanone A, and methylophiopogonanone B. METHODS Comatex C₁₈ column (4.6 mm??250 mm, 5 ??m)was used for the HPLC analysis. The mobile phase consisted of acetonitrile and water (58??42) and was eluted at the flow rate of 1 mL??min^-1. The column temperature was maitained at 30 ??. The detection wavelength was set at 280 nm. The injection volume was 15.0 ??L. RESULTS The linear regression equations of methylophiopogonone A, methylophiopogonanone A and methylophiopogonanone B were Y=493 321??+31 262(r=0.999 9), Y=605 744??+40 941(r=0.999 9), and Y=586 672??+39 657(r=0.999 9), respectively. The average recovery rates of the three flavones respectively were 100.59%(RSD=1.51%), 99.27%(RSD=1.28%), and 100.04%(RSD=1.33%). CONCLUSION The established method for simultaneous determination of flavone constituents in Ophiopogonis Radix is accurate and sensitive, with good repeatability. It can be applied to the quality evaluation of Ophiopogonis Radix.