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Determination and Quality Evaluation of Seven Chemical Components of Gardenia jasminoides Ellis

??OBJECTIVE To develop an UPLC method for simultaneous determination of seven components in Gardenia jasminoides, ie, gardoside, shanzhiside, deacetyl asperulosidic acid methyl ester, gardenoside (RG), genipin-1-??-D-gentiobioside, chlorogenic acid, and gardenoside to evaluate the quality of Gardenia jasminoides. METHODS ACQUITY UPLC HSS T3 column was used for the UPLC analysis. The mobile phase was acetonitrile-0.05% phosphoric acid solution. Gradient elution was conducted at a flow rate of 0.2 mL??min^-1. The column temperature was maitained at 30 ?? and detection wavelength was set at 238 nm. A linear model was obtained through principal component analysis (PCA), and the scores of the principal components were used to evaluate the quality of Gardenia jasminoides Alba decoction pieces comprehensively. RESULTS The seven components could be well separated from each other with good specificity, precision,repeatability,linearity,recovery rate and stability.The 25 Gardenia jasminoides Ellis samples and two Gardenia jasminoides Ellis var.grandiflora Nakai samples conformed to the quality requirements in the chapter of gardoside, shanzhiside, deacetyl asperulosidic acid methyl ester, gardenoside(RG), genipin-1-??-D-gentiobioside, chlorogenic acid, gardenoside. As the comprehensive evaluation shown, the quality of wild Gardenia jasminoides samples from Jiangxi province was better; Gardenia jasminoides from inland provinces excelled those from coastal provinces; and Gardenia jasminoides across Jiangxi province were of stable and higher quality. CONCLUSION The method established in this study can effectively assay geniposide, gardoside, shanzhiside, deacetyl asperulosidic acid methyl ester, gardenoside and genipin gentiobioside in Gardenia jasminoides, thus it can be used for the quality control of Gardenia jasminoides.