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Effects of Anti-Inflammatory Active Fraction from the Tuber of Scirpus yagaraohwi on Mice with Intracerebral Haemorrhage

??OBJECTIVE To study the effects of anti-inflammatory active fraction (AF) from the tuber of Scirpus yagaraohwi in mice with intracerebral haemorrhage(ICH). METHODS A 25 ??L volume of autologous nonanticoagulated blood was collected from the tail artery of the mouse and then injected into the striatum using a syringe pump. Neurological deficits of the mouse was assessed 1, 3 and 5 days after ICH using a 28-point neurological deficit scale, and brain water content of the mouse cerebral tissues after ICH was measured at third day. The concentrations of pro-inflammatory cytokines in perihematoma tissues, including interleukin-6, interleukin-1?? and tumor necrosis factor-??, were measured by ELISA assay. RESULTS The results show that AF significantly reduced the neurological deficit scores and the brain water content after ICH. AF also markedly inhibited the levels of IL-6, IL-1?? and TNF-?? at first day and third day after ICH. CONCLUSION Anti-inflammatory active fraction of Scirpus yagaraohwi can improve the brain injury in mouse induced by ICH. The mechanism may involve decreased expression of pro-inflammatory cytokines of mouse after ICH.