KGF对口腔黏膜上皮细胞中增殖相关基因PCNAmRNA的作用

目的：检测不同浓度角质细胞生长因子（KGF）对体外培养的口腔黏膜上皮细胞形态学及对上皮细胞增殖相关基因PCNAmRNA表达的影响。方法：体外培养的口腔黏膜上皮细胞加入含不同浓度KGF（0ng／mL，5ng／rnL，25ng／mL，50ng／mL）的D—KFSM，分别培养12h、24h、48h后观察细胞形态改变并用荧光实时定量检测各组细胞内增殖相关基因PCNAmRNA的表达。结果：①相同时间段实验组较对照组上皮细胞贴壁明显，培养48h实验3组（50ng／mL）较其他组细胞核仁明显；②12h时，实验组较对照组上皮细胞内PCNAmRNA表达增加，但各实验组间PCNAmRNA表达逐渐降低（P〈0．05）；③24h时实验组较对照组PCNAmRNA表达增加，但各实验组间无统计学差异护〉0．05）：④48h时，实验组较对照组PCNAmRNA表达增加，且呈剂量依赖性（P〈0．05）。结论：外源性KGF可上调口腔黏膜上皮细胞增殖相关基因PCNAmRNA的表达，且在不同时间段、不同浓度调控作用存在差异。

Study on the function of KGF on apoptosis of cultured oral mucosal epithelial cells

Objective：To detect the morphology and expression of PCNA mRNA of oral epithelial cells co-cultured with different concentrations of KGF. Method：Add the D-KFSM with different concentrations of KGF （0 ng/mL,5 ng/mL,25 ng/mL,50 ng/mL） in oral mucosal cells from the fifth to the tenth generation. The morphology change of cells co-cultured was observed after 12h, 24h and 48h respectively. Real-time PCR was used to detect the expressions of PCNA mRNA in each group after 12 h,24 h and 48 h respectively. Result： ①After co-cuhured with different concentrations of KGF,the cells in experimental groups were adherent more tightly and with enhanced shading than ceils in control group. Cells in experi- mental group 3 showed obvious nuclei at 48 h;② The expression level of PCNA mRNA in experimental groups was higher than control group（P 〈0.05） at 12h while the expression was decreased with the concentration of KGF increased（P 〈0.05）;③ The expression level of PCNA mRNA in experimental groups was higher than control group while there was no difference a- mong experimental groups at 24 h （P 〉0.05）; ④The expression level of PCNA mRNA in experimental groups was increased with concentration of KGF increased（P 〈0.05）. Conclusion：Exogenous KGF could increase the mRNA expression of PCNA in the oral epithelial cells to some extent depending on the concentration and interaction time.