蝎毒素对C57BL/6小鼠Lewis肺癌生长及免疫细胞的影响

目的:观察蝎毒素(ITX)对Lewis肺癌生长及对荷瘤小鼠免疫细胞的影响。方法:建立Lewis肺癌荷瘤小鼠模型后,随机分为模型组、rIL-2组、ITX组、空白组,每组8只小鼠,接种第2日起,每天腹腔注射给药,连用16天。末次给药24h后,用流式细胞术检测小鼠外周血中免疫细胞的功能状态;酶联免疫法检测小鼠外周血TGF-β1水平,并测量瘤体质量和体积。结果:ITX干预后荷瘤小鼠单核、NK和B细胞均有一定活化,但无统计学意义;rIL-2干预后荷瘤小鼠的NK和B细胞有明显活化(P<0.05);ITX组和rIL-2组血清TGF-β1水平均高于模型组,rIL-2组明显高于模型组(P<0.05);ITX和rIL-2干预后瘤体质量和体积均有增加,ITX组明显高于模型组(P<0.05)。结论:ITX和rIL-2均促进了瘤体质量和体积的增加,可能与其活化的免疫细胞有关。

Effect of Scorpion Venom on Tumor Growth and Immune Cells of Lewis Lung Carcinoma in C57BL/6 Mice

Objective:To investigate the effect of scorpion venom(ITX) on tumor growth and immune cells of Lewis Lung Carcinoma in C57BL/6 Mice.Methods:Holland Lewis lung carcinoma mice were taken as the model and randomly divided into four groups:the model group,the rIL-2 group,the ITX group,the normal control group.There were 8 mice in each group.Each group was inoculated intraperitoneally since the second day of inoculation for 16 days.Twenty-four hours after the last injection,the level of immune cells in peripheral blood was analyzed by flow cytometry;the contentence of TGF-β1 in peripheral blood serum was detected by enzyme-linked immune sorbent assay;subcutaneous tumors were measured for tumor weight and volume.Results:The activation of monocyte,NK and B cells were promoted by ITX,without statistics difference(P>0.05).The levels of NK and B cells were elevated by rIL-2(P<0.05).The level of TGF-β1 was elevated by ITX and rIL-2,but only rIL-2 group was statistical different(P<0.05).ITX and rIL-2 promoted tumor weight and volume,but only ITX group was statistical different(P<0.05).Conclusion:ITX and rIL-2 can promote tumor growth.It may be related to the activation of immune cells.