| 消斑通脉合剂对PDGF-BB诱导血管平滑肌细胞增殖的影响 |
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| 引用本文： | 武跃华,杨积武. 消斑通脉合剂对PDGF-BB诱导血管平滑肌细胞增殖的影响[J]. 实用中医内科杂志, 2010, 24(2): 9-10. DOI: 10.3969/j.issn.1671-7813.2010.02.04 |
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| 作者姓名： | 武跃华 杨积武 |
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| 作者单位： | 1. 辽宁中医药大学,辽宁沈阳,110032
2. 辽宁中医药大学附属医院,辽宁沈阳,110032 |
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| 摘 要： | [目的]观察中药复方消斑通脉合剂药物血清对体外培养的VSMCs细胞周期的作用。[方法]体外培养VSMCs,取对数生长期细胞用作实验,MTT法检测不同浓度药物血清对体外培养的VSMCs增殖活力;应用流式细胞仪分析各组细胞周期分布。[结果] MTT法显示药物血清呈剂量依赖性抑制VSMCs增值,高剂量与低剂量比较有统计学差异(P<0.05),流式细胞仪显示药物血清可明显阻滞VSMCs细胞进程,高剂量与低剂量比较有统计学差异(P<0.01)。[结论]中药复方消斑通脉合剂药物血清可以阻滞VSMCs的细胞周期进程,达到抑制VSMCs增殖的作用。
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| 关 键 词： | 血管平滑肌细胞 药物血清 细胞周期 |
Effect of XiaoBanTongMaiHeJi on Rat Vascular Smooth Muscle Cells Cycle in Vitro |
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| WU Yue-hua,YANG Ji-wu. Effect of XiaoBanTongMaiHeJi on Rat Vascular Smooth Muscle Cells Cycle in Vitro[J]. , 2010, 24(2): 9-10. DOI: 10.3969/j.issn.1671-7813.2010.02.04 |
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| Authors: | WU Yue-hua YANG Ji-wu |
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| Affiliation: | WU Yue - hua , YANG Ji - wu ( 1. Liaoning University of Traditional Chinese Medicine, Shenyang 110032 ,Liaoning, China ; 2. The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China) |
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| Abstract: | [ Objective] To study the anti -proliferation and cell cycle blocking effects of the xiao ban tong mai he ji on rat vascular smooth muscle cells（VSMCs） in vitro. [Methods] VSMCs were treated with various concentration of the medical serum of xiao ban tong mai he ji for 24 hours. The cell viability was detected by MTT method. Flow cytometry was used to detect the cell cycle. [ Results] Compared with the control group, the medical serum inhibited the proliferation of VSMCs. the cells numbers of G0/G1phase increased, and the ceils numbers of S phase decreased significantly. [ Conclusion] The medical serum of xiao ban tong mai he ji inhibit of the proliferation and blocks cell cycle progression of VSMCs. |
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| Keywords: | vascular smooth muscle cell drug serum cell cycle |
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