成釉细胞瘤和角化囊肿细胞生长特点的研究

目的观察、比较成釉细胞瘤和角化囊肿细胞的生长特点，探讨角化囊肿复发的原因。方法原代体外培养成釉细胞瘤细胞、牙源性角化囊肿、根端囊肿及口腔粘膜的上皮细胞和成纤维细胞，倒置光相差显微镜观察。结果成釉细胞瘤上皮细胞生长较快，细胞生长晕周围不规则，大细胞生长晕周围有许多卫星样小生长晕，成纤维细胞呈网格状。角化囊肿与其他囊肿和正常粘膜的上皮细胞相同，生长缓慢，细胞生长晕周围规则，由呈漩涡状生长的成纤维细胞围绕。结论成釉细胞瘤细胞生长活跃，而角化囊肿细胞的生长特点与其他囊肿和粘膜相似，提示成釉细胞瘤细胞的浸润性生长特点，推测角化囊肿复发的主要因素不是因为其具有肿瘤的生长特点。

Growth behavior of cells of ameloblastoma and odontogenic keratocyst

Objective To compare the growth behavior of epithelium and fibroblast from ameloblastoma and keratocyst and to discuss the recurrence reason for the keratocyst.Methods Epithelial cells and fibroblast from ameloblastoma, keratocyst, dentigerous cyst, radicular cyst and oral mucosa were cultured and observed by phase contrast microscope. Results The epithelial cells of ameloblastoma grew faster, with an irregular area around the cell mass and some small satellite shaped cell mass. The epithelial cells of all the cysts and mucosa grew slower with the cell masses surrounded by fibroblasts which grew in whirl manner with a round and smooth periphery. Conclusion The cells from the ameloblastoma grow faster and more active,and those from the keratocysts grow like the other cysts and the normal mucosa. It is speculated that the ameloblastoma grows in an aggressive manner and it is possible that the recurrence of the keratocyst isn't primarily resulted from its growth behavior as a tumor.