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正交试验优选荔枝核总皂苷提取工艺
引用本文:陈吉生,;黄雪莹. 正交试验优选荔枝核总皂苷提取工艺[J]. 中国药房, 2009, 0(27): 2107-2109
作者姓名:陈吉生,  黄雪莹
作者单位:[1]广东药学院附属第一医院,广东省广州市510080; [2]广州中医药大学,广东省广州市510006
摘    要:目的:优选荔枝核总皂苷的提取工艺。方法:以荔枝核总皂苷的含量和提取物收率为指标,以提取溶剂、溶剂用量、提取次数和提取时间为考察因素,采用正交试验法优选最佳提取工艺。结果:最佳提取工艺是以70%乙醇为提取溶剂,溶剂用量为1g药材粉末加入8mL溶剂,提取1次,每次1h。结论:此工艺简便、易行,稳定性好,可为进一步利用荔枝核提供理论参考。

关 键 词:荔枝核  总皂苷  提取工艺  正交试验

Optimization of the Extraction Technology of Total Saponins from Litchi chinensis by Orthogonal Experiment
Affiliation:CHEN Ji-sheng,HUANG Xue-ying(1.The First Affiliated Hospital of Guangdong College of Pharmacy, Guangzhou 510080, China;2.Guangzhou University of TCM,Guangzhou 510006,China)
Abstract:OBJECTIVE: To optimize the extraction technology of total saponins from Litchi chinensis .METHODS: The extraction technology of total saponins from Litchi chinensis was optimized by orthogonal experiment taking the content of total saponins and extraction rate as indexes with the extraction solvent, the amount of solvent, the extraction time and extraction times as factors. RESULTS: The optimal extraction technology of total saponins from Litchi chinensis was as follows: 7096 ethanol as solvent, 1g medicinal powder(per 8 mL solvent), extraction time of 1 hour (extraction for 1 time). CONCLUSION: The optimum extracting technology is simple, feasible and stable, and it provides theoretic basis for the further utilization of Litchi chinensis.
Keywords:Litchi chinensis  Total saponins  Extraction technology  Orthogonal experiment
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