正交试验优选蒸制黄芩的纤维素酶辅助提取工艺

目的:优选蒸制黄芩的纤维素酶辅助提取工艺。方法:以黄芩苷和汉黄芩苷的提取率之和为指标,以纤维素酶用量、温度、pH值、时间为考察因素,采用正交试验法优选最佳提取工艺。结果:最佳提取工艺为纤维素酶用量为15U.g-1药材,温度50℃,时间7h,pH4.8。在该提取条件下,黄芩苷和汉黄芩苷的提取率分别为8.44%和2.62%。结论:纤维素酶辅助提取法优于传统的水煎煮法,为提取黄芩苷和汉黄芩苷的有效工艺,可明显提高黄芩药材的利用率。

Optimization of Cellulase - assited Extraction Technology of the Steam - processed Scutellaria baicalensis by Orthogonal Experiment

OBJECTVIE： To optimize the cellulase- assisted extraction technology of steam processed Scutellaria baicalensis. METHODS： The cellulase- assisted extraction technology of steam- processed S.baicalensis was optimized by orthogonal experiment with the extraction rates of baicalin and wogonoside as indexes and with cellulase concentration, extracting temperature, extracting time and pH value as factors. RESLUTS： The optimal cellulase- assisted extraction technology of steam- processed S.baicalensis was as follows： the concentration of cellulase was 15 U·g^-1; the extracting tem- perature was 50 ℃ ; the extracting time was 7 h and the pH value was 4.8. Under the optimal conditions, the extraction rates of baicalin and wogonoside were 8.44% and 2.62% , respectively. CONCLUSION： The ceUulase- assisted extraction tech- nology of steam processed S.baicalensis is superior to the traditional water-decoction method, and it proves to be an effective process for the extraction of baicalin and wogonoside from steam- processed S.baicalensis and by which the utilization rate of S.baicalensis can be improved.