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| HPLC法测定对坐叶中木犀草素的含量 | |
| 引用本文： | 陈华国,;靳凤云,;赵超,;周欣,;龚小见. HPLC法测定对坐叶中木犀草素的含量[J]. 中国药房, 2009, 0(36): 2838-2839 |
| 作者姓名： | 陈华国, 靳凤云, 赵超, 周欣, 龚小见 |
| 作者单位： | [1]贵州师范大学天然药物质量控制研究中心,贵阳市550001; [2]贵州省山地环境信息系统与生态环境保护重点实验室,贵阳市550001; [3]贵阳中医学院,贵阳市550002 |
| 基金项目： | 贵州省中小企业发展专项资金计划（黔中小局发[2008]76号）;贵州省科技计划课题（黔科合带帽字[2008]5005号） |
| 摘 要： | 目的:建立以高效液相色谱法测定对坐叶中木犀草素含量的方法。方法:色谱柱为Diamonsil C18(250mm×4.6mm,5μm),流动相为甲醇-0.04%磷酸溶液(60∶40),流速为1mL.min-1,柱温为35℃,检测波长为350nm。结果:木犀草素进样量在101.6～812.8ng范围内与峰面积积分值呈良好线性关系(r=0.9999);平均回收率为97.32%,RSD=0.9%(n=9)。结论:本方法简便、准确、分离效果好,可用于对坐叶的质量控制。 |
| 关 键 词： | 对坐叶 木犀草素 高效液相色谱法 含量测定 |
Determination of Luteolin in Itedyotis uncinella by HPLC |
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| Affiliation: | CHEN Hua-guo, ZHAO Chao, ZHOU Xin, GONG Xiao-jian, JIN Feng-yun(1Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang 550001,China;2Guizhou Provincial Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guiyang 550001, China;3Guiyang College of TCM, Guiyang 550002, China) |
| Abstract: | OBJECTIVE： To determine the content of luteolin in Hedyotis uncinella by HPLC. METHODS： The chromatographic separation was performed on Diamonsil C18（250 mm ×4.6 mm, 5 μm） with column temperature kept at 35 ℃. The mobile phase consisted of acetonitrile - 0.04% phosphoric acid（60 ： 40） at a flow rate of 1 mL · min^-1; the detection wave- length was set at 350 nm. RESULTS： The linear range for luteolin was 101.6-812.8 ng （r = 0.999 9） with an average recovery of 97.32% （RSD = 0.9%, n = 9）.CONCLUSION： The method is simple and accurate with good results in sample separation, and it is applicable for the quality control of H.uncinella. |
| Keywords: | Hedyotis uncinella Luteolin HPLC Content determination |
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