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| HPLC法测定驱虫斑鸠菊中紫铆素的含量 | |
| 引用本文： | 范广建,张坚,孙力,于鲁海,尚靖,徐建国. HPLC法测定驱虫斑鸠菊中紫铆素的含量[J]. 中国药房, 2009, 0(30): 2366-2367 |
| 作者姓名： | 范广建 张坚 孙力 于鲁海 尚靖 徐建国 |
| 作者单位： | 新疆维吾尔自治区人民医院药剂科,乌鲁木齐市830001 |
| 摘 要： | 目的:建立以高效液相色谱法测定驱虫斑鸠菊中紫铆素含量的方法。方法:色谱柱为HypersilODS(250mm×4.6mm,5μm),流动相为甲醇-0.5%磷酸溶液(40∶60),检测波长为280nm。结果:紫铆素进样量在0.01～0.5mg范围内与峰面积积分值呈良好线性关系(r=0.9999);平均回收率为93.6%,RSD=2.89%(n=9)。结论:本法简单、准确,可用于驱虫斑鸠菊的质量控制。 |
| 关 键 词： | 驱虫斑鸠菊 紫铆素 高效液相色谱法 含量测定 |
Determination of Butin in Vernonia anthelmintica by HPLC |
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| FAN Guang-jian,ZHANG Jian,SUN Li,YU Lu-hai,SHANG Jing,XU Jian-guo. Determination of Butin in Vernonia anthelmintica by HPLC[J]. China Pharmacy, 2009, 0(30): 2366-2367 | |
| Authors: | FAN Guang-jian ZHANG Jian SUN Li YU Lu-hai SHANG Jing XU Jian-guo |
| Affiliation: | (Dept. of Pharmacy, Xin-jiang Autonomous Region People＇s Hospital, Urumqi 830001, China) |
| Abstract: | OBJECTIVE： To establish an HPLC method for determination of butin in Vernonia anthemintica . METHODS： The samples were determined on Hypersil ODS column（250mm×4.6mm,5μm）, the mobile phase consisted of methanol- 0.5% HaPO4 （40 ： 60） and the detection wavelength was set at 280 nm. RESULTS： The linear range of butin was 0.01-- 0.5 rag（ r = 0. 999 9） and the average recovery was 93.6% （RSD = 2.89%, n = 9） .CONCLUSION ： The method is simple and accurate,and it is applicable for the quality control of butin in V.anthelmintica. |
| Keywords: | Vernonia anthelmintica Butin HPLC Content determination |
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