内弹力膜损伤对新生内膜的影响

目的通过球囊扩张并拉伤兔腹主动脉建立血管损伤模型,分析血管损伤与新生内膜之间的关系,进一步探讨内弹力膜损伤对经皮冠状动脉介入术后再狭窄产生的影响。方法对32只雄性新西兰大耳白兔进行股动脉穿刺,经导丝送人球囊,球囊扩张并拉伤腹主动脉建立血管损伤模型。术后28天处死模型兔,分离损伤血管,进行固定、包埋及染色处理。使用计算机图像分析软件测量新生内膜厚度,分析确定血管损伤积分。结果血管损伤积分与新生内膜厚度呈正相关。血管损伤积分与新生内膜厚度之间的拟合曲线为呈"S"形的三次曲线。结论内弹力膜损伤加速新生内膜增生。

The Influence of Internal Elastic Membrane Injury on Newborn Neointima

Aim To establish the vascular injury model through balloon dilation and strain abdominal aorta in rabbits; To analyze the relationship between vascular injury and neointima; To investigate the effect of internal elastic membrane injury on restenosis after percutaneous coronary intervention. Methods The femoral artery puncture was performed in 32 male New Zealand white rabbits, through lead wire feeding balloon. Blood vessel injury model was established by balloon dilatation and strain of abdominal aorta. After 28 days, the rabbits were sacrificed and the injured blood vessels were isolated. The fixation, embedding and staining were performed. Computer image analysis software was used to measure the thickness of the newborn neointima, and to determine the integral of vascular injury. Results The integral of vascular injury was positively correlated with the thickness of newborn neointima. The fitting curve between the integral of vascular injury and the thickness of newborn neointima was the S-shaped cubic curve. Conclusion Internal elastic membrane injury accelerates intimal hyperplasia.