Short-coherence off-axis holographic phase microscopy of live cell dynamics |
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| Authors: | Stefan Witte Andrius Plau?ka Margreet C. Ridder Laura van Berge Huibert D. Mansvelder Marie Louise Groot |
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| Affiliation: | 1These authors contributed equally to this work;2LaserLaB, VU University Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands;3Department of Child Neurology, VU University Medical Centre, 1081 HV Amsterdam, The Netherlands;4Department of Integrative Neurophysiology, CNCR, VU University, Amsterdam, Netherlands;5Neuroscience Campus Amsterdam, VU University, 1081 HV Amsterdam, The Netherlands |
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| Abstract: | ![]()
We demonstrate a single-shot holographic phase microscope that combines short-coherence laser pulses with an off-axis geometry. By introducing a controlled pulse front tilt, ultrashort pulses are made to interfere over a large field-of-view without loss of fringe contrast. With this microscope, quantitative phase images of live cells can be recorded in a full-field geometry without moving parts. We perform phase imaging of HEK293 cells, to study the dynamics of cell volume regulation in response to an osmotic shock.OCIS codes: (110.0110) Imaging systems, (090.1995) Digital holography, (170.1650) Coherence imaging, (320.7160) Ultrafast technology, (170.1530) Cell analysis |
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