Ascorbic acid prevents ischemia-reperfusion injury in the rat small intestine |
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Authors: | M Nakamura Michitaka Ozaki Shohei Fuchinoue Satoshi Teraoka Kazuo Ota |
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Institution: | (1) Department of Surgery III, Tokyo Women's Medical College, 8–1, Kawada-cho, Shinjuku-ku, Tokyo 162, Japan, JP |
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Abstract: | Ischemia-reperfusion injury by free radicals and lipid peroxides is observed in various organs. Ascorbic acid (AsA) or glutathione
(GSH) in various doses (AsA:2, 0.5, 0.1 mmol/kg, GSH:2 mmol/kg) was intraperitoneally administered to male Wistar rats. The
entire small intestines were resected just before ischemia, after ischemia, and after 20 min of reperfusion (n = 7–10 at each time point). At each time point, the specimens were subjected to assays of lipid peroxides, GSH, and glutaminase
activity of the tissues; they were also examined histologically. In the AsA group, the production of lipid peroxides after
reperfusion was significantly suppressed in a dose-dependent manner, and the ratio of oxidized GSH to total GSH was also significantly
low. Tissue glutaminase activity decreased to a lesser extent, and the degree of injury was apparently less marked in the
AsA group. This study indicates that AsA acts as an antioxidant against peroxidative tissue injury, possibly by scavenging
radicals, preserving reduced GSH, and reducing the peroxidative reaction.
Received: 21 June 1996 Received after revision: 8 October 1996 Accepted: 12 November 1996 |
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Keywords: | Small intestine ascorbic acid reperfusion Ascorbic acid small intestine reperfusion Reperfusion ascorbic acid small intestine Ischemia ascorbic acid reperfusion |
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