软肝化坚颗粒对C57小鼠肝纤维化模型肝功能及转化生长因子β_1的影响

目的观察软肝化坚颗粒对肝纤维化模型C57小鼠肝功能及转化生长因子β1(TGF-β1)的影响。方法将60只C57小鼠随机分为6组,即正常对照组、正常软肝化坚颗粒组、模型对照组、模型软肝化坚颗粒低剂量组、模型软肝化坚颗粒高剂量组和模型秋水仙碱组,每组10只。各模型组通过腹腔注射10%四氯化碳(CCl4)造模,造模2周后各用药组灌胃软肝化坚颗粒或秋水仙碱,用药6周时采集血清,测定小鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)及TGF-β1的含量。结果模型对照组小鼠血清ALT、AST及TGF-β1的含量均高于正常对照组(P0.05),模型软肝化坚颗粒低剂量组、模型软肝化坚颗粒高剂量组以及模型秋水仙碱组小鼠血清ALT、AST的含量与正常对照组相比明显增高(P0.05),正常软肝化坚颗粒组小鼠血清ALT含量与正常对照组相比降低(P0.05),与模型对照组相比则明显降低(P0.05),模型软肝化坚颗粒高剂量组血清ALT、AST水平低于模型秋水仙碱组(P0.05)。模型软肝化坚颗粒组(包括低剂量和高剂量组)、模型秋水仙碱组和正常软肝化坚颗粒组小鼠血清TGF-β1的含量与模型对照组相比均明显降低,且均降低至正常对照组水平(P0.05)。结论软肝化坚颗粒可以改善肝功能,抑制TGF-β1的产生,对实验性肝纤维化有一定的预防和治疗作用。

Effect of Ruangan Huajian granule on liver function and serum TGF-β1 level in C57 mice with experimental hepatic fibrosis

Objective To study of the effect of Ruangan Huajian Granule on liver function and serum TGF-β1 in C57 mice with experimental hepatic fibrosis.Methods 60 mice were randomly divided into six groups,normal control group,normal Ruangan Huajian Granule group,model control group,model Ruangan Huajian Granule low dose group,model Ruangan Huajian Granule high-dose group and model colchicine group.There were 10 mice in every group.C57 mice with experimental hepatic fibrosis were induced by intraperitoneal injection of 10% CCl4.Ruangan Huajian Granule and colchicine was applied different groups.The levels of serum ALT,AST and TGF-β1 were detected in the six groups.Results ALT,AST and TGF-β1 in model control group were higher than those of normal control group（P〈0.05）.The levels of serum ALT,AST and TGF-β1 in model Ruangan Huajian Granule low dose group,model Ruangan Huajian Granule high-dose group and model colchicine group were notably lower than those of model control group（P〈0.05）.The levels of ALT and AST in model Ruangan Huajian Granule high-dose group was lower than those of model colchicine group（P〈0.05）.Conclusion s Ruangan Huajian Granule can improve liver function and inhibit the production of TGF-β1.It is of definite therapeutic and preventive value for mice with experimental hepatic fibrosis.