| 正交试验法优选化橘红中肌醇提取工艺 |
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| 引用本文： | 陈地灵,陈振兴,林励,吴泽青,吴俊生.正交试验法优选化橘红中肌醇提取工艺[J].广州中医药大学学报,2012,29(4):424-427. |
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| 作者姓名： | 陈地灵 陈振兴 林励 吴泽青 吴俊生 |
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| 作者单位： | 广州中医药大学中药学院,广东广州,510006 |
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| 基金项目： | 国家科技部"十二五"科技支撑计划项目 |
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| 摘 要： | 【目的】优选化橘红中肌醇最佳提取工艺。【方法】以乙醇浓度、料液比和提取温度为考察因素,采用高效液相—蒸发光散射检测器(HPLC-ELSD)色谱法测定化橘红中肌醇含量,采用正交试验法优选化橘红中肌醇最优提取工艺。【结果】化橘红中肌醇最佳提取工艺为体积分数70%乙醇、料液比1∶20、提取温度100℃、每次时间45 min、提取2次。【结论】本研究优选得到的提取工艺稳定、提取率高,可用于化橘红中肌醇的提取。
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| 关 键 词： | 化橘红/化学 肌醇/分析 正交试验 提取工艺 色谱法 高压液相 |
Optimization of Extraction Technology for Inositol from Exocarpium Citri Grandis |
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| CHEN Diling
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CHEN Zhenxing
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LIN Li
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WU Zeqing
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WU Junsheng.Optimization of Extraction Technology for Inositol from Exocarpium Citri Grandis[J].Journal of Guangzhou University of Traditional Chinese Medicine,2012,29(4):424-427. |
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| Authors: | CHEN Diling CHEN Zhenxing LIN Li WU Zeqing WU Junsheng |
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| Institution: | (School of Chinese Herbal Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510006 Guangdong,China) |
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| Abstract: | Objective To optimize the extraction technology for the inositol from Exocarpium Citri Grandis.Methods With ethanol concentration,the ratio of material to solvent and extracting temperature as factors,the extraction technology for the inositol from Exocarpium Citri Grandis was optimized by orthogonal design and through the determination of inositol content by high performance liquid chromatography-evaporative light scattering detector(HPLC-ELSD).Results The optimal extraction technology was as follows: 70% ethanol,the ratio of material to solvent being 1∶ 20,extraction at 100 ℃ for 45 min and extraction twice.Conclusion The extraction technology is steady,extraction percentage is higher and the technology is suitable for the extraction of inositol from Exocarpium Citri Grandis. |
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| Keywords: | EXOCARPIUM CITRI GRANDIS/chemistry INOSITOL/analysis ORTHOGONAL DESIGN EXTRACTION TECHNOLOGY CHROMATOGRAPHY HIGH PRESSURE LIQUID PHASE |
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