肝硬化患者肠道菌群的初步分析及肠型分析

目的初步对肝硬化与肠道菌群的关系进行探讨并对肝硬化患者肠道菌群进行简单的肠型分析。方法纳入符合诊断标准的肝硬化患者80例,并选取同期健康志愿者40名。采用粪便细菌基因组DNA提取试剂盒采集肝硬化患者及健康志愿者的粪便,应用16s rDNA测序技术检测肠道菌群,并应用Qiime流程以及R软件等对测序结果进行分析。结果随着肝硬化的发生及严重程度的增加,致病菌如Escherichia/Shigella(埃希氏菌属/志贺氏菌属)、Streptococcus(链球菌属)、Veillonela(韦荣菌属)明显增加,而有益菌Prevoseburia(罗斯拜瑞氏菌属)、f_;achnospiraceae(毛螺旋菌科下的未知属)明显减少。其中,Escherichia/Shigella(埃希氏菌属/志贺氏菌属)的平均相对丰度百分比与Child-Pugh评分呈显著正相关。肠道菌群结构以Escherichia(埃希氏菌属)为主的人群罹患肝硬化且发展为重度肝硬化的风险更高。结论肝硬化的发生发展与肠道菌群密切相关且以Escherichia为主的肠道菌群结构为肝硬化的危险肠型。

Analysis of intestinal flora in patients with liver cirrhosis

Objective To investigate the relationship between liver cirrhosis and intestinal flora and to perform simple enterotypes of intestinal flora in patients with liver cirrhosis. Methods 80 patients diagnosed as liver cirrhosis and 40 healthy volunteers selected for the same period were included. A fecal bacterial genomic DNA extraction kit was used to collect fecal and the intestinal flora was detected by 16 s rDNA sequencing technology whose result was analyzed by Qiime software, R software and so on. Results With the occurrence and the severity of liver cirrhosis, the mean relative abundance percentage of some species of intestinal flora changed. Pathogenic bacteria such as Escherichia/Shigella, Streptococcus and Veillonela increased significantly, while beneficial bacteria such as Prevose-buria and f;sub>Lachnospiraceae decreased significantly. The mean relative abundance percentages of Escherichia/Shigella showed a significant positive correlation with Child-Pugh scores in patients with cirrhosis. And people with a intestinal flora structure predominated by Escherichia were at higher risk of developing severe cirrhosis. Conclusion The occurrence and development of cirrhosis are closely related to intestinal flora and Escherichia predominant intestinal flora structure is a dangerous enterotype of cirrhosis.