乌司他丁对创伤性脑损伤的保护作用研究

目的探讨乌司他丁(UTI)对创伤性脑损伤的保护作用和具体机制。方法 BALB/c小鼠27只,随机分为空白组(shame组)、生理盐水对照组(control组)、损伤组(TBI组)和乌司他丁预处理组(UTI组)。利用小鼠神经功能评分(NSS)法判断乌司他丁的治疗作用;Western blot方法检测脑组织中cathepsin B蛋白表达变化情况。结果与shame组比较,TBI组的cathepsin B蛋白表达明显增加(P<0.05),UTI组cathepsin B蛋白表达显著减少(P<0.05);与空白组比较,UTI组能明显减轻神经功能障碍,各组间差异有统计学意义(P<0.05)。结论乌司他丁预处理可能通过调控cathepsin B减轻细胞死亡,从而有效改善脑损伤后神经功能,具有显著的神经保护作用。

Protective effects of ulinastatin on mice with traumatic brain injury

Objective To investigate the potential therapeutic effect and the related mechanism of ulinastatin （UTI） on mice with traunmtic brain injury. Methods A total of 27 BALB/c mice were randomly divided into four groups ：sham group, TBI group, physiological saline control group and the UTI pretreatment group （UTI group）. Neurological functions of mice were treasured by nerve function score （NSS） ; Western blot was applied to detect the expression of cathepsin B protein of brain tissues. Results There was significant difference in the expression of cathepsin B among sham group, TBI group and UT/group （P 〈0. 05）. The expression of cathepsin B protein was decreased obviously in UTI group （P 〈0.05）. Conclusion The UTI pretreatment may reduce the cell death by regu- lating cathepsin B protein, which thereby can effectively improve the neurological functions following TBI.