AGT、ACE基因多态性与成人过敏性紫癜和过敏性紫癜性肾炎的相关性

目的 探讨肾素-血管紧张素系统中AGT基因M235T和ACE基因I/D多态性与过敏性紫癜和过敏性紫癜性肾炎易感性的关系.方法 应用PCR和PCR - RFLP技术检测145例过敏性紫癜/过敏性紫癜性肾炎患者与172例正常对照组血管紧张素原基因第2外显子M235T多态性及血管紧张素转换酶基因第16内含子I/D多态性.结果 ①AGT基因型构成比在HSP组、HSPN组与正常对照组之间差异有统计学意义(P=0.008,P=0.002),但在HSP和HSPN之间差异无统计学意义(P =0.180).AGT- TT基因型和T等位基因携带者具有较高的患HSP、HSPN的风险.②ACE基因型构成比在HSPN组与正常对照组之间差异有统计学意义(P=0.003),但在HSP和正常对照组、HSP和HSPN之间差异无统计学意义(P=0.065,P=0.073).ACE - DD基因型和D等位基因携带者具有较高的患HSPN的风险.结论 携带AGT基因M235T多态性增加患HSP/HSPN的风险,携带ACE基因I/D多态性增加患HSPN的风险.

Association of AGT and ACE gene polymorphism with genetic susceptibility to Henoch-Schonleinpurpura and Henoch-Schonleinpurpura nephritis of adult

Objective To explore M235 T polymorphism of angiotensinogen （AGT） gene and I/D polymorphism of angiotensin converting enzyme（ACE） gene in renin-angiotensin system, and their rela- tionship with Henoch-Sehonlein p＇urpura （ HSP ）/Henoch- Schonleinpurpura nephritis （ HSPN ）. Methods Insertion/deletion（I/D） polymorphism in intron 16 of ACE gene and number two exon M235T polymor- phisms of AGT gene were analyzed in 90 patients with HSP/HSPN and 98 healthy adult controls, by using PCR-RFLP and PCR. Results （1）The AGT genotype distribution was significantly different between HSP and normal control, and between HSPN and normal control （ P = 0. 008 , P = 0. 002 ） , but no difference was observed between HSP and HSPN （ P = 0. 180 ） . The AGT gene TT genotype and T allele has a high risk for HSP and HSPN. （2）The ACE genotype distribution was significantly different between HSPN and normal control （ P = 0. 003 ） , but no difference was observed between HSP and normal control, neither between HSP and HSPN（ P = 0. 065 ,P = 0. 073 ）. The ACE gene DD genotype and D allele has a high risk for HSPN. Conclusion AGT gene M235T polymorphism is associated with HSP/ HSPN. ACE gene I/D polymorphism is associated with HSPN.