姜黄及其3种近源品种的姜黄素类成分薄层色谱鉴别及HPLC特征图谱分析

目的：鉴别分析姜黄及其3种近源品种（蓬莪术、温莪术、广西莪术）的姜黄素类成分。方法：以姜黄对照药材，姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素对照品为对照进行薄层色谱鉴别；以姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素对照品为对照物质，采用HPLC对10批姜黄进行特征图谱研究，建立HPLC特征图谱共有模式，进行相似度比较，并将姜黄与3种近源品种进行相似度比对。色谱条件：采用月旭AQ-C18（200mm×4．6mm，5μm）色谱柱，以乙腈-水为流动相进行梯度洗脱，流速1mL·min^-1，检测波长270nm，柱温30℃。结果：供试品色谱中，姜黄与3种莪术的斑点位置、颜色、数量有明显差别；建立了姜黄的HPLC特征图谱，确定了7个共有峰，10批姜黄药材样品的平均相似度在0.97，姜黄与3种近源品种的相似度均低于0．8，特征图谱差异明显。结论：姜黄及其3种近源品种的薄层色谱及HPLC特征图谱差异明显，且方法简便、准确、专属性强，可作为姜黄及其3种近源品种的鉴别，同时也为姜黄对照药材标定技术标准的建立提供了科学依据。

Analysis Curcuminoids in Curcumae Longae Rhizoma and Its Three Near-source Species by TLC Identification and HPLC Fingerprint Chromatogram

Objective：Researching Curcumae longae rhizoma and its three near-source species and ldentificate the kinds of the curcuminoids, netherds： TLC was used to identificate curcuminoids in Curcumae longae rhizoma and its three near-source species, with Curcumae longae rhizoma, Curcumine, Demethoxycurcumin, Diplo-demethoxycurcumin as reference substance. We established a HPLC fingerprint chromatogram to identificate the difference from near-source species. Results：The distinction of Curcumae longae rhizoma, and its three near-source species are obviously difference and can be identificated by TLC and HPLC fingerprint chromatogram. Conclusion： The identification methods were proved to be available and effective.