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| HPLC测定光果甘草中光甘草定的含量 | |
| 引用本文： | 林灵超,阮佳佳. HPLC测定光果甘草中光甘草定的含量[J]. 中药研究与信息, 2012, 0(6): 4-6 |
| 作者姓名： | 林灵超 阮佳佳 |
| 作者单位： | [1]浙江福立分析仪器有限公司,浙江温岭317500 [2]温岭第三人民医院,浙江温岭317523 |
| 摘 要： | 目的：采用HPLC测定光果甘草粗黄酮浓缩物中光甘草定的含量。方法：以FL2200-II为高效液相色谱仪，采用UltimateXB—C。8色谱柱，以水和乙腈为流动相，进行梯度洗脱，检测波长280nm，流速1．0mL·rain-1。结果：光甘草定在9．5～475txg·mL。内线性良好，r=0．9998；测得某光果甘草粗黄酮浓缩物中光甘草定的平均含量为3．58％，RSD=1．23％，平均加标回收率99．04％，RSD=0．26％。结论：利用反相高效液相色谱对甘草粗黄酮浓缩物中的光甘草定进行含量测定，方法简便，准确度高，重现性好，可应用于光甘草定的含量测定。 |
| 关 键 词： | HPLC 光果甘草 光甘草定 梯度洗脱 |
HPLC determinated the content of Glabridin from Glycyrrhiza glabra L. |
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| LIN Ling-chao,RUAN Jia-jia. HPLC determinated the content of Glabridin from Glycyrrhiza glabra L.[J]. Research & Information on Traditional Chinese Medicine, 2012, 0(6): 4-6 | |
| Authors: | LIN Ling-chao RUAN Jia-jia |
| Affiliation: | 1. ZheJiang Fuli Analytical Instrumentation Co. Ltd, Wenling 317500, China ; 2. Third People＇ s Hospital of Wenling, Wenling 317523, China) |
| Abstract: | Objective：To establish a method for the determination of Glabridin in crude flavonoid concentrate of Glycyrrhiza glabra L. with high-performance liquid chromatography FL2200-II. Methods： A ultimate XB-Cls column was used with mobile phase （ gradient elution） being water and acetonitrile, at the flow rate of 1.0 mL. rain - , detecting wavelength of 280 nm.. Results： Linearities was good at 9.5 - 475 μg. mL - l （ r = 0. 999 8 ）. The average recovery rate was 99.04% （ RSD = 0. 26% ）. The average content of glabridin in the crude flavonoid concentrate from Glycyrrhiza glabra L. was 3.58% （n = 6）. Conclusion：The method, is simple and easy, can be used to determinate Glabridin in Glycyrrhiza glabra L. |
| Keywords: | HPLC Glycyrrhiza glabra L. Glabridin Gradient elution |
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