双酚A暴露对大鼠器官结构及细胞因子表达的影响

目的研究双酚A暴露对大鼠器官结构及细胞因子表达的影响。方法F344大鼠按体重随机分为对照组和低、中、高剂量组,分别给予0、4、40和400mg/kg双酚A,观察脾脏、胸腺、肝脏、肾脏组织形态学的改变,实时定量PCR测量脾脏IL-2、IL-12、IFN-γ、TNF-α和胸腺Fas、FasL六种细胞因子mRNA表达的改变。结果与对照组相比,高剂量组体重降低(P<0.05),脾脏白髓减少,脾小体缩小,肾小球缩小,肝脏出现肝细胞浑浊变性和纤维化,其它指标无明显改变。与对照组相比,各剂量组脾脏IL-2、IL-12、IFN-γ、TNF-α mRNA表达均显著降低(P<0.01),胸腺Fas表达下降(P<0.01),FasL表达在低剂量组出现一个短暂的下降后(P<0.05),在中、高剂量组明显上升(P<0.01)。结论双酚A,可改变靶器官的生长、发育及生理功能,影响细胞因子的表达,进而影响机体免疫功能。

Effect of bisphenol A on the structure of organs and the expression of some cytokines in rats

Objective To study effect of bisphenol A on the structure of organs and the expression of some cytokines of spleens in F344 rats.Methods Fisher 344 rats were divided into control,low-dose,middle-dose and high-dose groups,and were gavaged to 4mg/kg,40mg/kg,400mg/kg bisphenol A per day,respectively. To observe the change of histomorphology of spleens,thymus,kidneys and livers. To detect the mRNA expression of IL-2,IL-12,IFN-γ,TNF-α in spleen and Fas,FasL in thymus by real-time RT-PCR.Results Contrast to control,the weight of high dose group decreased(P<0.05).In 400mg/kg group,white pulp of spleens reduced ,splenic corpuscle contracted ,granular degeneration was visible in hepatocyte,and tubular atrophy was occurred in some kidneys of rats. But there were not any change in others groups. The mRNA expression of IL-2,IL-12,IFN-γ,TNF-α in all groups decreased significantly(P<0.01). The expression of Fas decrease in all treatment groups but the expression of FasL up-regulated in middle and high dose groups after it decrease in low dose group(P<0.05).Conclusion The environmental endocrine disruptor such as bisphenol A has the same chemical structure as hormone,they can change the development and the function of target organs,possible influence the expression of some cytokines and the immune function.