| HPLC法测定人参提取物中人参皂苷Re和人参皂苷Rg1的含量 |
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| 引用本文： | 黄新兴,梁廷. HPLC法测定人参提取物中人参皂苷Re和人参皂苷Rg1的含量[J]. 中国中医药现代远程教育, 2011, 9(21): 131-132. DOI: 10.3969/j.issn.1672-2779.2011.21.084 |
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| 作者姓名： | 黄新兴 梁廷 |
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| 作者单位： | 1. 吉林省通化市食品药品检验所,通化,134000
2. 吉林紫鑫药业股份有限公司,柳河,135300 |
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| 摘 要： | 目的研究高效液相色谱法测定人参提取物中人参皂苷Re和人参皂苷Rg1。方法采用反相高效液相色谱法,KNAUER-C18(250mm×4.6mm,5μm)柱,乙腈:1%磷酸溶液(20:80)为流动相,流速为1.0ml·min-1,检测波长203nm,柱温为30℃,进样量10μl。结果人参皂苷Re和人参皂苷Rg1的平均回收率分别为100.2%和98.8%。结论该方法简便、准确、重复性好,可用于人参提取物中人参皂苷Re和人参皂苷Rg1的含量测定。
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| 关 键 词： | HPLC 人参皂苷Re 人参皂苷Rg1 含量测定 |
Determination of Ginsenoside Re and Ginsenoside Rg1 in Panax Ginseng C.A.Extractions by RP-HPLC |
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| Huang Xinxing,Liang Ting. Determination of Ginsenoside Re and Ginsenoside Rg1 in Panax Ginseng C.A.Extractions by RP-HPLC[J]. Chinese Medicine Modern Distance Education of China, 2011, 9(21): 131-132. DOI: 10.3969/j.issn.1672-2779.2011.21.084 |
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| Authors: | Huang Xinxing Liang Ting |
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| Affiliation: | Huang Xinxing1,Liang Ting2(1.Tonghua Food and Drug Administration of Jilin,JilinTonghua,134000,2.Jilin Zinxin Pharmaceutical Co.Ltd.,JilinLiuhe,135300) |
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| Abstract: | Objective To determine the content of ginsenoside Re and ginsenoside Rg1 in panax ginseng C.A.extractions.Method A HPLC method was developed,The chromatographic conditions were as follows,KNAUER-C18（250mm×4.6mm,5μm） column.The mobile phase was Acetonitrile-1% Phosphoric acid solution（20：80）.The detection wavelength at 203nm.Results The ginsenoside Re and ginsenoside Rg1＇s average recovery were 100.2% and 98.8%.Conclusion The method is simple,accurate and with good repeata.And this method can be used for the determination of the content of ginsenoside Re and ginsenoside Rg1 in panax ginseng C.A.extractions. |
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| Keywords: | HPLC Ginsenoside Re Ginsenoside Rg1 Content determination |
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