| DIFA检测非放射性斑点印迹技术的探讨 |
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| 引用本文： | 郑乃刚,丁一,吴景兰,苏安英,丰艳,王一菱.DIFA检测非放射性斑点印迹技术的探讨[J].郑州大学学报(医学版),1996(1). |
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| 作者姓名： | 郑乃刚 丁一 吴景兰 苏安英 丰艳 王一菱 |
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| 作者单位： | 河南省临床检验中心，河南医科大学分子细胞生物学研究中心，邯郸医学院组织学胚胎学教研室 |
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| 摘 要： | 为提高分子生物学的实验效率和节省昂贵的试剂，将核酸分子或蛋白质分子预先结合于硝酸纤维素膜（ＮＣＭ）上，应用自制的ＤＩＦＡ装置检测非放射性斑点印迹。结果表明：此技术可快速检测生物活性试剂的效价及工作浓度；选择研究样本及试验条件；鉴定探针灵敏度等。
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| 关 键 词： | 免疫斑点渗滤试验 斑点印迹 非放射性标记信号 |
Application of DIFA to non-radioactive dot blot technique |
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| Zheng Haigang, Ding Yi, Wu Jinglan, Su Anying, Feng Yan, Wang Yiling.Application of DIFA to non-radioactive dot blot technique[J].Journal of Zhengzhou University: Med Sci,1996(1). |
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| Authors: | Zheng Haigang Ding Yi Wu Jinglan Su Anying Feng Yan Wang Yiling |
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| Abstract: | In order to promote the experimental efficiency of molecular biology technique and save expensive reagents, the nucleic acid molecules or protein molecules were precoated onto the nitrocellulose membrane(NCM), and the non-radioactive labelled signal was detected by DIFA (dot immunofiltration assay) equipment prepared in our laboratory. The results showed that this method could be used for detection of the titre and working dilution of biologicallyactive reagents, selection of research samples,optimal. experimental conditions and identification of probe sensitivity. |
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| Keywords: | dot immunofiltration assay dot blot non-radioactive labeled signal |
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