| Preparation of ChIL-2 and IBDV VP2 Fusion Protein by Baculovirus Expression System |
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| 引用本文： | Yan Liu~(1,2) Yongwei Wei~2 Xiaofeng Wu~3 Lian Yu~(2,4) ~1Department of Biomedical Engineering,Zhejiang University,Hangzhou,310027,China,~2Zhejiang Province Key Lab of Preventive Veterinary Medicine,China,~3College of Animal Sciences,Zhejiang University,Huajiachi Campus,Hangzhou 310029,China,~4Corresponding to:Dr.Lian Yu,Institute of Preventive Veterinary Medicine,Zheiiang University,268 Kai Xuan Road,Hangzhou 310029,China.. Preparation of ChIL-2 and IBDV VP2 Fusion Protein by Baculovirus Expression System[J]. Cellular & molecular immunology, 2005, 0(3) |
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| 作者姓名： | Yan Liu~(1 2) Yongwei Wei~2 Xiaofeng Wu~3 Lian Yu~(2 4) ~1Department of Biomedical Engineering Zhejiang University Hangzhou 310027 China ~2Zhejiang Province Key Lab of Preventive Veterinary Medicine China ~3College of Animal Sciences Zhejiang University Huajiachi Campus Hangzhou 310029 China ~4Corresponding to:Dr.Lian Yu Institute of Preventive Veterinary Medicine Zheiiang University 268 Kai Xuan Road Hangzhou 310029 China. |
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| 作者单位： | Yan Liu~(1，2) Yongwei Wei~2 Xiaofeng Wu~3 Lian Yu~(2，4) ~1Department of Biomedical Engineering，Zhejiang University，Hangzhou，310027，China;~2Zhejiang Province Key Lab of Preventive Veterinary Medicine，China;~3College of Animal Sciences，Zhejiang University，Huajiachi Campus，Hangzhou 310029，China;~4Corresponding to:Dr.Lian Yu，Institute of Preventive Veterinary Medicine，Zheiiang University，268 Kai Xuan Road，Hangzhou 310029，China. |
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| 摘 要： | This study aims to produce an effective subunit vaccine against infectious bursal disease virus (IBDV).The genes ofchicken interleukin-2 (ChIL-2) and IBDV viral protein 2 (VP2) were amplified and fused by splice overlapextension-polymerase chain reaction (SOE-PCR).The fusion gene was digested by EcoR I/Kpn I and inserted intopBacPAK8 vector,resulting in recombinant transfer plasmid pBacPakVP2-IL2.The recombinant plasmid wastransfected into Sf-9 cells accompanied with hybrid nuclear polyhedrosis virus (HyNPV) genome DNA andlipofectin.Plaque-purification indicated that we had got the recombinant Hy-VP2-IL2.Fusion protein VP2-IL2was expressed effectively both in insect cells and bombyx mori.The expression of fusion protein was confirmed byELISA,SDS-PAGE and Western blotting assay,respectively.This efficient system allows us to meet the need forinexpensive vaccines required by the poultry industry.Cellular & Molecular Immunology.2005;2(3):231-235.
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Preparation of ChIL-2 and IBDV VP2 Fusion Protein by Baculovirus Expression System |
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| Yan Liu. Preparation of ChIL-2 and IBDV VP2 Fusion Protein by Baculovirus Expression System[J]. Cellular & molecular immunology, 2005, 0(3) |
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| Authors: | Yan Liu |
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| Abstract: | This study aims to produce an effective subunit vaccine against infectious bursal disease virus (IBDV).The genes of chicken interleukin-2 (ChIL-2) and IBDV viral protein 2 (VP2) were amplified and fused by splice overlap extension-polymerase chain reaction (SOE-PCR).The fusion gene was digested by EcoR I/Kpn I and inserted into pBacPAK8 vector,resulting in recombinant transfer plasmid pBacPakVP2-IL2.The recombinant plasmid was transfected into Sf-9 cells accompanied with hybrid nuclear polyhedrosis virus (HyNPV) genome DNA and lipofectin.Plaque-purification indicated that we had got the recombinant Hy-VP2-IL2.Fusion protein VP2-IL2 was expressed effectively both in insect cells and bombyx mori.The expression of fusion protein was confirmed by ELISA,SDS-PAGE and Western blotting assay,respectively.This efficient system allows us to meet the need for inexpensive vaccines required by the poultry industry.Cellular & Molecular Immunology.2005;2(3):231-235. |
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| Keywords: | IBDV ChIL-2 fusion gene fusion protein |
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