Comparison of Tetrahymena ARS sequence function in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe |
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| Authors: | Kenneth R. Luehrsem Ronald E. Pearlman Janice Pata Eduardo Orias |
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| Affiliation: | (1) Department of Biological Sciences, University of California, 93106 Santa Barbara, CA, USA;(2) Department of Biology, York University, 4700 Keele Street, MM 1P3 Toronto, Ontario, Canada;(3) Present address: Department of Biological Sciences, Stanford University, 94305 Stanford, CA, USA |
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| Abstract: | Summary We have isolated several Tetrahymena thermophila chromosomal DNA fragments which function as autonomously replicating sequences (ARS) in the heterologous Saccharomyces cerevisiae and Schizosaccharomyces pombe selection systems. The Tetrahymena ARS sequences were first isolated in S. cerevisiae and were derived from non-ribosomal micro- and macronuclear DNA. Sequence analysis of the ARS elements identified either perfect or close matches with the 11 by S. cerevisiae ARS core consensus sequence. Subcloning studies of two Tetrahymena ARS elements defined functional regions ranging in size from 50 to 300 bp. Testing of the ARS elements in S. pombe revealed that most of the T. thermophila inserts confer ARS function in both yeasts, at least in the sense of promoting a high transformation frequency to plasmids which contain them. However, the actual sequences responsible for ARS activity were not always identical in the two yeasts. |
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| Keywords: | ARS Saccharomyces cerevisiae Schizosaccharomyces pombe Tetrahymena thermophila |
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