Difference in the ability of HLA serum to react with T and B lymphocyte populations

The ability of HLA sera to react with T and B lymphocytes of human blood was studied. The lymphocytes were separated by removal of one of the cell populations. The method of rosette formation was used, followed by centrifugation in a density gradient and adsorption of B lymphocytes on synthetic fiber. After removal of the B cells the cytotoxic activity of the HLA sera was reduced. Removal of T lymphocytes did not affect the result of the lymphocytotoxic test. It is postulated that B lymphocytes contain more determinants of HLA antigens than T lymphocytes.Laboratory of Immunohematology, Central Institute of Hematology and Blood Transfusion, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR P. N. Kosyakov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 81, No. 6, pp. 698–699, June, 1976.