Recognition and utilisation of dansyl-dipeptides in manual dansyl-Edman sequencing

The chromatographic behaviour of the dansyl-dipeptides likely to be encountered in manual dansyl-Edman sequencing is presented. The dansyl dipeptides were either encountered in experimental sequencing or chemically synthesised and in some cases both. The advantages of using short hydrolysis times and deliberately generating these dipeptides are discussed.