A NIR fluorescent probe for Vanin-1 and its applications in imaging,kidney injury diagnosis,and the development of inhibitor |
| |
| Institution: | 1. Dalian Key Laboratory of Metabolic Target Characterization and Traditional Chinese Medicine Intervention, The Second Affiliated Hospital, College of Integrative Medicine, College of Pharmacy, Dalian Medical University, Dalian 116044, China;2. Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, China;3. School of Life Sciences, Northwestern Polytechnical University, Xi''an 710072, China;4. School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China;5. State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China;6. College of Pharmacy, Key Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Hangzhou Normal University, Hangzhou 311121, China |
| |
| Abstract: | Vanin-1 is an amidohydrolase that catalyses the conversion of pantetheine into the amino-thiol cysteamine and pantothenic acid (coenzyme A precursor), which plays a vital role in multiple physiological and pathological processes. In this study, an enzyme-activated near-infrared (NIR) fluorescent probe (DDAV) has been constructed for sensitively detecting Vanin-1 activity in complicated biosamples on the basis of its catalytic characteristics. DDAV exhibited a high selectivity and sensitivity toward Vanin-1 and was successfully applied to the early diagnosis of kidney injury in cisplatin-induced kidney injury model. In addition, DDAV could serve as a visual tool for in situ imaging endogenous Vanin-1 in vivo. More importantly, Enterococcus faecalis 20247 which possessed high expression of Vanin-1 was screened out from intestinal bacteria using DDAV, provided useful guidance for the rational use of NSAIDs in clinic. Finally, oleuropein as a potent natural inhibitor for Vanin-1 was discovered from herbal medicines library using a high-throughput screening method using DDAV, which held great promise for clinical therapy of inflammatory bowel disease. |
| |
| Keywords: | Vanin-1 NIR fluorescent probe Kidney injury diagnosis Fluorescence imaging Oleuropein |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
