强直性脊柱炎患者HLA-B27和免疫指标检测的临床意义

目的:探讨HLA-B27和免疫指标在强直性脊柱炎(AS)患者的临床意义.方法:采用流式细胞术检测147例AS患者、95例正常对照者的HLA-B27、CD3 T、CD4 T、CD8 T、CD19 B,用免疫比浊法检测IgA、IgG和IgM.结果:AS患者HLA-B27、CD3 T、CD4 T淋巴细胞的百分率及CD4 /CD8 的比值、血清IgA、IgG均明显高于正常对照组(P＜0.01);CD8 T淋巴细胞的百分率低于正常对照组(P＜0.05);CD19 B淋巴细胞的百分率高于正常对照组(P＜0.05);IgM与正常对照组相比无显著性差异.结论:HLA-B27、CD3 T、CD4 T与AS密切相关,提示,HLA-B27与CD3 T、CD4 T、CD4 /CD8 的比值可作为AS辅助诊断指标.

Clinical Significance of Determination of HLA-B27 and Immuno-Markers in Patients with Ankylosing Spondylitis

Objective To explore the significance of presence of changes HLA-B27 and of other immuno-markers in patients with ankylosing spondylitis(AS). Methods HLA-B27, CD3 T, CD4 T, CD8 T, CD19 B positive rate (with FCM), serum IgA, IgG, IgM levels (with immuno-turbidity) were determined in 147 patients with AS as well as in 95 controls. Results CD3 T lymphocyte, CD4 T lymphocyte positive rate and the ratio of CD4 /CD8 , serum IgA, IgG levels were significantly higher in patients than those in the controls (P<0.01). The CD19 B lymphocyte positive rate was also higher in patients than that in controls (P<0.05), the CD8 T lymphocyte positive rate was lower in patients that in controls (P<0.05). The serum IgM level was not significantly different (vs controls, P>0.05). Conclusion HLA-B27, CD3 T, CD4 T contents were closely related to development of AS. HLA-B27, CD3 T, CD4 T, CD4 /CD8 could be used as diagnostic indicators for AS.