骨骼肌肿瘤中c—myc癌基因的改变及其预后意义

探讨ｃ－ｍｙｃ癌基因在骨骼肌肿瘤中的改变及其预后意义。方法，运用Ｓｏｕｔｈｅｒｎ杂交和ＲＮＡ打点杂交技术分析１８例骨骼肌肿瘤及相对应瘤组织中ｃ－ｍｙｃ癌基因的发迹并对术后患者进行３年随访。结论：ｃ－ｍｙｃ癌基因以扩增，重排及高表达方式参与骨骼肌肿瘤的发生发展，检测有无ｃ－ｍｙｃ癌基因的异常可辅助判断骨骼肌肿瘤的预后。

Alteration of c myc oncogene in musculoskeletal tumors and its prognostic significance

Objective: The purpose of the study was to explore effects of c myc oncogene on progression of musculoskeletal malignant tumors. Methods: By way of Southern blot and RNA dot blot, alterations of c myc oncogene were analysed in 18 samples of musculoskeletal tumors and corresponding peritumorous tissues. Then the patients were followed up for 3 years after operation. Results: Six samples were found for amplification by Southern blot, of which there were 3 samples of osteosarcomas accompanied by rearrangement, 1 sample of malignant fibrous histocytoma, 1 sample of glandular alveolus soft tissue sarcoma, 1 sample of para tumor tissue. Ten samples of tumors were found for overexpression of c myc oncogene by RNA dot blot as well as 2 samples in peritumorous tissues. One sample of peritumorous tissues with overexpression was corresponded to tumor with amplification, the other was with amplification of itself and its corresponding tumor tissue. In 10 tumor patients with amplification and rearrangement or overexpression of c myc , 8 died within 3 years, 3 patients at 0.5 year, 4 patients at 1 year, and 1 patient at 1.5 years after operation; 2 patients without c myc alteration died at 2 years and 3 years respectively, the others still survived. There was significant difference of 3 year survival rate between group of normality of c myc and group of alteration of c myc ( P <0.05). Conclusion: c myc oncogene takes part in progression of musculoskeletal tumors by way of amplification rearrangement and overexpression, screening alteration of c myc existence in musculoskeletal tumors may be an useful prognostic predicator.