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Identification of IY81149 and its metabolites in the rat plasma using the on-line HPLC/ESI mass spectrometry |
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| Authors: | Seung-Woon Myung Hye-Ki Min Changbae Jin Myungsoo Kim Seung Mok Lee Gi Ju Chung Seong Jun Park Dong Yeon Kim Hyun-Woo Cho |
| Institution: | Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, Seoul, Korea. swmyung@kist.re.kr |
| Abstract: | Reversed-phase high-performance liquid chromatography/mass spectrometry (HPLC/MS) with an electrospray ionization (ESI) interface was applied to the identification of metabolites of IY 81149 in the rat plasma. Fragments obtained using collision-induced dissociation (CID) in both positive and negative modes were utilized to elucidate the structure of metabolites. The eluent from the conventional HPLC column was split and directly introduced into an ESI-mass spectrometer for the identification of the structures. The CID technique allowed the sensitive identification of sulfonyl-IY81149 and hydroxy-IY81149 from the rat plasma. |
| Keywords: | IY81149 Metabolites H+/K+-ATPase inhibitor HPLC Electrospray ionization (ESI) Mass spectrometry (MS) Collision induced dissociation (CID) |
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