| HPLC法测定云南白药胶囊中三七皂苷R_1和人参皂苷Rg_1的含量 |
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| 引用本文： | 钱鑫,徐飞. HPLC法测定云南白药胶囊中三七皂苷R_1和人参皂苷Rg_1的含量[J]. 西北药学杂志, 2013, 28(3) |
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| 作者姓名： | 钱鑫 徐飞 |
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| 作者单位： | 1. 广东省佛山市药品检验所,佛山,528000
2. 宁夏回族自治区疾病预防控制中心,银川,750004 |
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| 摘 要： | 目的采用高效液相色谱法测定云南白药胶囊中三七皂苷R1和人参皂苷Rg1含量。方法色谱条件:Phenomenex C18柱(100mm×4.6mm,2.6μm);流动相:乙腈-水(19∶81);流速:1mL.min-1;柱温:30℃;检测波长:203nm。结果三七皂苷R1和人参皂苷Rg1的线性范围分别为0.53～3.17μg(r=0.999 8),2.27～13.62μg(r=0.999 8);回收率(n=6)分别为100.6%(RSD=2.1%),97.7%(RSD=1.8%)。结论该方法准确可靠,重复性好,可用于测定云南白药胶囊中三七皂苷R1和人参皂苷Rg1含量。
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| 关 键 词： | 云南白药胶囊 三七皂苷R1 人参皂苷Rg1 高效液相色谱 含量测定 |
Determination of notoginsenoside R1 and ginsenoside Rg1 in Yunnan Baiyao Capsules by HPLC |
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| QIAN Xin , XU Fei. Determination of notoginsenoside R1 and ginsenoside Rg1 in Yunnan Baiyao Capsules by HPLC[J]. Northwest Pharmaceutical Journal, 2013, 28(3) |
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| Authors: | QIAN Xin XU Fei |
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| Abstract: | Objective To establish an HPLC method for the determination of notoginsenoside R1and ginsenoside Rg1in Yunnan Baiyao Capsules.Methods The chromatographic separation was performed on a Phenomenex C18column(100mm×4.6mm,2.6 μm),the mobile phase was acetonitrile-water(19∶81),the flow rate was 1mL.min-1 at 30℃,and the detection wavelength was 203nm.Result The results show that the linear ranges for notoginsenoside R1and ginsenoside Rg1were 0.53-3.17μg(r=0.999 8),and 2.27-13.62μg(r=0.999 8),and the average recoveries were 100.6%(RSD=2.1%)and 97.7%(RSD=1.8%)respectively(n=6).Conclusion The method is accurate,reliable and reproducible,and could be used as a quality control method for notoginsenoside R1and ginsenoside Rg1in Yunnan Baiyao Capsules. |
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| Keywords: | Yunnan Baiyao Capsules notoginsenoside R1 ginsenoside Rg1 HPLC determination |
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