INF-γ/A+874T位点基因多态性与呼吸道合胞病毒毛细支气管炎的相关性

目的：干扰素-γ(IFN-γ)分泌不足可能在严重呼吸道合胞病毒(RSV)感染的发病中发挥重要作用。IFN-γ/A+874T基因位点存在多态性，可能与RSV感染的病情存在关联。该研究分析INF-γ/A+874T基因位点在温州地区汉族儿童的分布特征；进一步探讨INF-γ/A+874T位点基因多态性与RSV毛细支气管炎易感性、病情的相关性及对鼻咽分泌物 INF-γ和血清总IgE水平的影响。方法：采用DNA测序法检测114例住院的汉族RSV毛细支气管炎患儿和90例汉族健康体检儿童INF-γ/A+874T位点基因多态性；采用酶联免疫吸附法测定鼻咽分泌物INF-γ水平，化学发光法测定血清总IgE水平。结果：两组均存在INF-γ/A+874T位点基因多态性，以AA纯合子基因型为主，其中RSV毛细支气管炎组AA、AT基因型频率分别为82.5%，17.5%，对照组分别为AA 77.8%，AT 21.1%，两组差异无显著性意义；病例组等位基因频率分别为A 90.4%，T 9.6%，对照组分别为A 88.3%，T 11.7%，两组差异均无显著性意义。轻度和中重度患儿INF-γ/+874位点2种基因型的差异亦无显著性意义。病例组2种基因型鼻咽分泌物INF-γ及血清总IgE水平差异均无显著性意义。结论：温州地区汉族儿童存在INF-γ/A+874T位点基因多态性，但未发现该位点变异与RSV毛细支气管炎易感性、病情、鼻咽分泌物INF-γ及血清总IgE水平存在关联。［中国当代儿科杂志，2009，11（1）：21-24］

Association of INF-γ/A+874T gene polymorphisms with respiratory syncytial virus bronchiolitis

Objective A deficient interferon-gamma (IFN-gamma) response has been involved in the pathogenesis of severe respiratory syncytial virus (RSV) infection. Gene polymorphisms in IFN-gamma/A+874T have been associated with the susceptibility to asthma and might be related to disease severity of RSV infection. This study investigated the single nucleotide polymorphisms (SNPs) of IFN-gamma/A+874T in Han children in Wenzhou area and to explore the correlation between gene polymorphisms of IFN-gamma/A +874T and the ...