FATE/BJ-HCC-2基因转染对肿瘤细胞生物学行为的影响

目的:观察FATE/BJ-HCC-2基因对肿瘤细胞生物学行为的影响,探讨FATE/BJ-HCC-2与肿瘤进展之间的关系。方法:将FATE/BJ-HCC-2基因转染肝癌细胞系Bel-7402细胞,3H-TdR掺入法和Transwell实验对转染细胞的增殖和侵袭能力进行检测;PCR方法检测细胞内的骨桥蛋白(OPN)和整合素连接激酶(ILK)基因的表达水平。结果:细胞增殖实验结果显示FATE/BJ-HCC-2转染肿瘤细胞的3H-TdR掺入值显著高于Mock细胞的掺入值,统计学分析二者差别有显著性意义(P<0.01)。FATE/BJ-HCC-2基因表达促进肝癌细胞系Bel-7402细胞的增殖,并且这种增殖效应可被特异性抗-FATE/BJ-HCC-2抗体所阻断。Transwell实验显示,转染FATE/BJ-HCC-2的细胞,其穿膜数量明显比对照组多,差别有显著的统计学意义(P<0.01)。对肿瘤细胞转移相关的细胞信号分子进行检测发现,骨桥蛋白(OPN)和整合素连接激酶(ILK)基因的表达水平上调。结论:肿瘤细胞获得FATE/BJ-HCC-2基因表达后其增殖和侵袭能力增强,FATE/BJ-HCC-2基因表达可能与肿瘤细胞的转移有一定关系。提示CT抗原的表达不仅仅是肿瘤发生中的伴随产物,它可能在肿瘤的发生发展过程中起到一定作用。

Effects of FATE/BJ-HCC-2 gene transfection on biological behavior of tumor cells

Objective:To elucidate the role of FATE/BJ-HCC-2 gene in tumorigenesis and cancer cell metastasis and its effects on biological behavior of tumor cells.Methods: The FATE/BJ-HCC-2 gene was stably transfected into HCC cell line Bel-7402 cells.The cell proliferation and invasive ability were detected with -thymidine uptake and transwell migration assays.mRNA expression of osteopontin(OPN) and integrin linked kinase(ILK) was analysed in FATE/BJ-HCC-2 gene-transfected cells by PCR.Results: The cell proliferation assay showed that -thymidine uptake in FATE/BJ-HCC-2 gene transfected tumor cells was signicantly higher than that in mock control cells.Statistical analysis indicated that the difference is significant(P<0.01).FATE/BJ-HCC-2 gene transfection enhanced Bel-7402 cells' proliferation.Importantly,this FATE/BJ-HCC-2 enhanced proliferation could be specifically blocked by anti-FATE/BJ-HCC-2 antibody.The result of transwell migration assay showed that the number of penetrated cells in the FATE/BJ-HCC-2 gene-transfected group was much more than that in control group.The difference between two groups is significant(P<0.01).Subsequent studies examining tumor metastasis associated molecules showed that mRNA expression of osteopontin(OPN) and integrin linked kinase(ILK) was upregulated in FATE/BJ-HCC-2 gene-transfected cells.Conclusion: The FATE/BJ-HCC-2 gene-transfected cells appear to have more potent proliferation and invasive ability and the expression of FATE/HCC-2 gene is related to metastasis of tumor cells.In general,our discoveries imply that cancer/testis(CT) antigens may play critical roles in tumorigenesis and tumor progression.