重楼皂甙Ⅰ对肺腺癌细胞株PC9增殖及凋亡的影响

目的]评价重楼皂甙Ⅰ对肺腺癌细胞株PC9增殖和凋亡的影响.方法]以体外培养的肺腺癌细胞株PC9为研究对象,MTT法检测重楼皂甙Ⅰ对PC9细胞增殖的抑制作用,流式细胞仪检测重楼皂甙Ⅰ对PC9细胞周期的影响,Annexin-V-FITC/PI双染法检测重楼皂甙Ⅰ对PC9细胞凋亡的影响,Western blot法检测重楼皂甙Ⅰ对PC9细胞Bcl-2、Bax、caspase-3蛋白表达的影响.结果]不同浓度重楼皂甙Ⅰ能有效抑制PC9细胞的增殖,且呈时间浓度依赖性(P＜0.01).2.5.μg/ml重楼皂甙Ⅰ作用PC9细胞12h、24h、48h后,出现G2/M期阻滞.2.5μg/ml重楼皂甙Ⅰ作用PC9细胞24h、48h后,细胞凋亡率明显增加,与对照组相比,具有统计学差异(P＜0.01).2.5μg/ml重楼皂甙Ⅰ作用PC9细胞48h后,Bcl-2蛋白表达降低、Bax及caspase-3蛋白表达增加,与对照组相比,亦具有统计学差异(P＜0.01).结论]重楼皂甙Ⅰ能抑制PC9细胞的体外增殖,且抑制作用表现出时效和量效关系,其机制可能与G2/M期阻滞、促进细胞凋亡、降低Bcl-2蛋白表达、增加Bax及caspase-3蛋白表达有关.

The Effect of Chonglou Saponin I on Proliferation and Apoptosis in Lung Adenocarcinoma Cell Line PC9

Purpose] To investigate the effect of Chonglou Saponin I on proliferation and apoptosis in lung adenocarcinoma cell line PC9.Methods] The effect of Chonglou Saponin I on cell prolifera-tion,cell cycle and cell apoptosis were detected by MTT method,FCM and Annexin-V-FITC/PI respectively.The expressions of Bcl-2,Bax and caspase-3 were detected by Western blot.Results] The growth of PC9 cells was inhibited by Chonglou Saponin I in a dose-and time-dependent manner.After treated with 2.5μg/ml Chonglou Saponin I 12h,24h and 48h,cell cycle was arrested in the G2/M phase.Compared with control group,cell apoptosis was induced significantly(P<0.01) after treated with 2.5μg/ml Chonglou Saponin I at 24h and 48h.The expression of Bcl-2 protein decreased,and the expressions of Bax and caspase-3 protein increased after treated with 2.5μg/ml Chonglou Saponin I at 48h.Conclusion] Chonglou Saponin I can inhibit cell prolife-ration of lung adenocarcinoma cell line PC9 in a dose-and time-dependent manner.The mechanism might relate to G2/M arrested,inducing cell apoptosis,decreasing expression of Bcl-2,increasing expression of Bax and caspase-3.